Anti-Mouse PD-1-BX027 (RMP1-30); Cy5-RX027

Pricing & Details

Product No.P410
Clone
RMP1-30
Protein
PD-1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Programmed Death-1, CD279, PD 1
Isotype
Rat
IgG2a κ
Applications
CODEX®
,
IHC FF
Prod No.
Size
Price
Avail.
Qty
Add to cart
P410-25 Tests
25 tests
$400.00
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Max:
Min: 1
Step: 1

CODEX® Details

Barcode
BX027
Reporter
Cy5-RX027
Fluorescent Dye
Cy5 is in the bright red fluorescent channel with an excitation max of 649 nm and an emission max of 666 nm.
Tissue Screened
MCA Sarcoma (Tumor)
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Mouse PD-1 transfected BHK cells
Formulation
This CODEX® barcoded antibody is formulated in phosphate buffered saline (150 mM NaCl) PBS, EDTA pH 7.2 containing 0.09% sodium azide as a preservative. The CODEX® reporter is lyophilized and formulated in 1X Tris-EDTA (TE) pH 8.0 (10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0)
Product Preparation
Manufactured in an animal-free facility using only in vitro cell culture techniques, purified by affinity chromatography, and conjugated to a specific barcode under optimal conditions.
State of Matter
CODEX® barcoded antibody - Liquid; CODEX® reporter - Lyophilized
Storage and Handling
This CODEX® barcoded antibody is stable when stored at 2-8°C for up to 1 year (do not freeze). The CODEX® reporter is stable when frozen at -20°C for up to 1 year.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX®; IHC FF - The suggested dilution for staining tissue in immunohistochemistry - Fresh Frozen (FF) is 1 μl of Anti-Mouse PD-1 (BX027) in a final volume of 200 μl of CODEX® staining buffer.
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone RMP1-14 recognizes an epitope on mouse PD-1.
Antigen Distribution
PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells.
Background
Antibody PD-1 is a 50-55 kD member of the B7 Ig superfamily. PD-1 is also a member of the extended CD28/CTLA-4 family of T cell regulators and is suspected to play a role in lymphocyte clonal selection and peripheral tolerance. The ligands of PD-1 are PD-L1 and PD-L2, and are also members of the B7 Ig superfamily. PD-1 and its ligands negatively regulate immune responses. PD-L1, or B7-Homolog 1, is a 40 kD type I transmembrane protein that has been reported to costimulate T cell growth and cytokine production. The interaction of PD-1 with its ligand PD-L1 is critical in the inhibition of T cell responses that include T cell proliferation and cytokine production. PD-L1 has increased expression in several cancers. Inhibition of the interaction between PD-1 and PD-L1 can serve as an immune checkpoint blockade by improving T-cell responses In vitro and mediating preclinical antitumor activity. Within the field of checkpoint inhibition, combination therapy using anti-PD1 in conjunction with anti-CTLA4 has significant therapeutic potential for tumor treatments. PD-L2 is a 25 kD type I transmembrane ligand of PD-1. Via PD-1, PD-L2 can serve as a coinhibitor of T cell functions. Regulation of T cell responses, including enhanced T cell proliferation and cytokine production, can result from mAbs that block the PD-L2 and PD-1 interaction.

CODEX® reporters are made up of a fluorescent dye and a short oligonucleotide DNA barcode called a CODEX® Tag. Fluorescent reporters enable highly specific detection of corresponding barcodes, and the use of spectrally separated dyes allow for precise signal detection in up to three distinct fluorescence channels at one time.

Antigen Details

Protein
Ligand/Receptor
PD-L1 (B7-H1), PD-L2
Function
Lymphocyte clonal selection, peripheral tolerance
PubMed
NCBI Gene Bank ID

References & Citations

1. Ardolino, M. et al. (2018) J Clin Invest. 128(10):4654-4668. PubMed
2. Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117.
3. Honjo, T. et al. (1992) EMBO J. 11:3887.
IHC

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.