Anti-Mouse/Rat CD126 (IL-6Rα) – Purified in vivo GOLD™ Functional Grade

Anti-Mouse/Rat CD126 (IL-6Rα) – Purified in vivo GOLD™ Functional Grade

Product No.: I-1185

[product_table name="All Top" skus="I-1185"]

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Clone
D7715A7 (15A7)
Target
CD126
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IL-6 Receptor α chain, IL-6R
Isotype
IgG2b κ
Applications
FA
,
FC
,
in vivo
,
N
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
OKT4 hybridoma cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this D7715A7 (15A7) antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this D7715A7 (15A7) antibody for use in western blotting is 1-10 μg/ml.
Additional Reported Applications For Relevant Conjugates ?
N
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone D7715A7 (Clone 15A7) recognizes an epitope on mouse CD126.
Background
CD126 (IL-6Rα) is an 80 kD type I cytokine receptor α chain that is a member of the Ig superfamily that can form a heterodimer with IL-6Rβ chain to form the high affinity IL-6 receptor. The IL-6/IL-6R complex is a potent pleiotropic cytokine that mediates cell growth and differentiation and has significant involvement in immune response. Inadequate production of IL-6 and IL-6R are thought to play a part in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer.
Antigen Distribution
CD126 is expressed on activated T and B cells, monocytes, hepatocytes and plasma cells.

Antigen Details

NCBI Gene Bank ID
Research Area
Cell Biology
.
Immunology
.
Innate Immunity
.
Neuroinflammation
.
Neuroscience
.
Signal Transduction
.
Other Molecules
FA
Flow Cytometry
in vivo Protocol
N
General Western Blot Protocol

Certificate of Analysis

Formats Available

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.