OKT4 hybridoma cells
≥ 5.0 mg/ml
< 1.0 EU/mg as determined by the LAL method
≥95% monomer by analytical SEC
>95% by SDS Page
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this D7715A7 (15A7) antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this D7715A7 (15A7) antibody for use in western blotting is 1-10 μg/ml.
Additional Reported Applications For Relevant Conjugates ?
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Clone D7715A7 (Clone 15A7) recognizes an epitope on mouse CD126.
CD126 is expressed on activated T and B cells, monocytes, hepatocytes and plasma cells.
CD126 (IL-6Rα) is an 80 kD type I cytokine receptor α chain that is a member of the Ig superfamily that can form a heterodimer with IL-6Rβ chain to form the high affinity IL-6 receptor. The IL-6/IL-6R complex is a potent pleiotropic cytokine that mediates cell growth and differentiation and has significant involvement in immune response. Inadequate production of IL-6 and IL-6R are thought to play a part in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer.
NCBI Gene Bank ID
Products are for research use only. Not for use in diagnostic or therapeutic procedures.