Anti-Phosphotyrosine Antibody (56494)
Anti-Phosphotyrosine Antibody (56494)
Product No.: 56494
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Clone G104 Target Phosphotyrosine Formats AvailableView All Product Type Monoclonal Isotype Mouse IgG1 Applications IHC , IP , WB , ICC/IF |
Data
Immunohistochemistry analysis using Mouse Anti-Phosphotyrosine Monoclonal Antibody, Clone G104 (56494). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Phosphotyrosine Monoclonal Antibody (56494) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Stratum granulosum staining in the epidermis. Some dermal staining.
Western Blot analysis of Human A431 cell lysates showing detection of Phosphotyrosine protein using Mouse Anti-Phosphotyrosine Monoclonal Antibody, Clone G104 (56494). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Phosphotyrosine Monoclonal Antibody (56494) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Left: normal, right: EGF treated. - -
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Antibody DetailsProduct DetailsReactive Species All Host Species Mouse Immunogen Phosphotyrosine, alanine and glycine in a 1:1:1 ratio polymerized in the presence of KLH with 1-ethyl-3-(3'- dimethylaminopropyl) carbodiimide. Product Concentration 1.0 mg/ml Formulation PBS, pH 7.4 State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1ug/mL.
Immunohistochemistry: use at 1-10ug/mL. Immunofluorescence: use at 1-10ug/mL. Positive control: Rat tissue lysate These are recommended concentrations; Enduser should determine optimal concentrations for their applications.See specific product references below for protocols and more information. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Monoclonal Antibody specific to Phosphotyrosine Background Protein phosphorylation is an important posttranslational modification that serves many key functions in regulation of protein activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by specific protein kinases that remove a phosphate group from ATP and attach it covalently to a recipient protein. Some kinases act on serine and threonine, others act on tyrosine, and many act on all three. Phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity. Antibodies to phosphotyrosine are useful in identifying protein substrates of tyrosine kinase. Research Area Neuroscience References & CitationsGarton AJ et al. 1996 Mol Cell Biol 16: 6408-6418. Garton AJ and Tonks NK 1999 J Biol Chem 274: 3811-3818. Technical Protocols   ICC/IF Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
