Anti-TRAP1/Hsp75 Antibody (11136)
Anti-TRAP1/Hsp75 Antibody (11136)
Product No.: 11136
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Clone Trap1-6 Target TRAP1/Hsp75 Formats AvailableView All Product Type Monoclonal Alternate Names HSP 75, TNFR-associated protein 1, Tumor necrosis factor type 1 receptor-associated protein, TRAP-1 Isotype Mouse IgG2a Applications WB , ICC/IF |
Data
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Trap1 Monoclonal Antibody, Clone Trap1-6 (11136). Tissue: PC3M cells. Species: Human. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody (11136) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Mitotracker (red) mitochondria stain. DAPI (blue) nuclear stain. (A) staining of mitochondria with MitoTracker dye; (B) staining of nuclei with DAPI; (C) staining with TRAP1 antibody; (D) tricolor composite of images A-C.
Western Blot analysis of Human Heat Shocked cervical cancer cell line (HeLa) lysate showing detection of Trap1 protein using Mouse Anti-Trap1 Monoclonal Antibody, Clone Trap1-6 (11136). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody (11136) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. - -
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Antibody DetailsProduct DetailsReactivity Species Human Host Species Mouse Immunogen Recombinant human TRAP1 Product Concentration 1.0 mg/ml Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezethaw cycles.
Regulatory Status For in vitro investigational use only. Not intended
for diagnostic or therapeutic applications. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1ug/mL. A band of ~75kDa is detected.
Immunocytochemistry: use at 0.1-1.0ug/mL. Immunofluorescence: use at 1-10ug/mL. Positive control: HeLa cells or lysate These are recommended concentrations; Enduser should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human TRAP1. Other species have not been investigated. Background The molecular chaperone family of 90kDa heat shock proteins (Hsp90) is a highly conserved protein family that plays an important physiological role. Hsp90 is best known for its association with signal transduction processes. Tumor necrosis factor receptor-associated protein 1 (TRAP1) is a recently cloned homolog of Hsp90. Like Hsp90, TRAP1 is associated with numerous proteins that are involved in multiple pathways. Data indicates that TRAP1 is a mitochondrial matrix protein: it is localized in mitochondria of mammalian cells and contains a mitochondrial targeting presequence. Antigen DetailsFunction Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. {PubMed:23525905, PubMed:23564345, PubMed:23747254}. NCBI Gene Bank ID UniProt.org Research Area Heat Shock & Stress Proteins References & CitationsTechnical Protocols ICC/IF |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
