Anti-tsp23 Antibody (11137)
Anti-tsp23 Antibody (11137)
Product No.: 11137
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Clone tsp232A Target tsp23 Formats AvailableView All Product Type Monoclonal Isotype Mouse IgG1 Applications IHC , WB , AM |
Data
Immunohistochemistry analysis using Mouse Anti-Tsp23 Monoclonal Antibody, Clone TSp232A (11137). Tissue: Heart Skeletal Muscle. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Tsp23 Monoclonal Antibody (11137) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Western Blot analysis of Human Heat Shocked cervical cancer cell line (HeLa) lysate showing detection of Tsp23 protein using Mouse Anti-Tsp23 Monoclonal Antibody, Clone TSp232A (11137). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Tsp23 Monoclonal Antibody (11137) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. - -
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Antibody DetailsProduct DetailsReactivity Species Human/Mouse Host Species Mouse Immunogen Recombinant tsp23 Product Concentration 1.0 mg/ml Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezethaw cycles. Regulatory Status For in vitro investigational use only. Not
intended for diagnostic or therapeutic
applications. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1ug/mL. A band of ~19kDa is detected.
These are recommended concentrations; Enduser should determine optimal concentrations for their applications. Positive control: Lysate of HeLa cells transiently transfected with Tsp23. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human and
mouse tsp23. Other species have not
been investigated. It does not crossreact with p23. Background Tsp23 (transcript similar p23) is a homolog of human p23, a highly conserved ubiquitous protein and a cochaperone for the Hsp90 chaperone system. A small protein (18-25kDa) with a simple structure, p23 is a highly acidic phosphoprotein with an aspartic acid- rich C-terminal domain. Although numerous studies have found p23 associated with other proteins (i.e, Fes tyrosine kinase, heme regulated kinase HRI, Hsf1 transcription factor, aryl hydrocarbon receptor, telomerase, and Hepadnavirus reverse transcriptase), the functional significance of p23 remains to be clearly defined. It appears to be involved in ATP-mediated Hsp90 binding of client proteins. A recent study suggests that p23 might be a promising target in leukemic apoptosis. tsp23 is 44% homologous with p23. Antigen DetailsNCBI Gene Bank ID UniProt.org Research Area Heat Shock & Stress Proteins References & CitationsTechnical Protocols  AM |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
