EL4 Whole Cell Lysate

EL4 Whole Cell Lysate

Product No.: E153

[product_table name="All Top" skus="E153"]

Product Type
Cell Line Lysate

Product Size


Cell Line
EL4 (T Lymphocyte; Lymphoma)
Growth Media
DMEM & 10% horse serum (Equine Serum)
EL4 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.