Human Growth Hormone MICRO-ELISA Test Kit

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Product Type
ELISA Kit
Alternate Names
somatotropin
Applications
ELISA
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T175-96 tests
96 tests
$340.00
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Product Details

Description
The MICRO-ELISA GROWTH HORMONE test is a solid phase sandwich-type enzyme immunoassay (ELISA) Diagnostic Kit for the In vitro quantitative determination of human growth hormone (hGH) concentration in human serum.
Materials Provided
Components in Each 96-Test Micro-Elisa hGH Diagnostic Kit

1. 96 wells, hGH ANTIBODY COATED WELLS: Coated with anti-hGH (rabbit polyclonal); contained in a pack with silica gel desiccant.
2. 1 bottle, 12.0 mL, hGH ENZYME ANTIBODY CONJUGATE: anti-hGH (mouse monoclonal) labeled with horseradish peroxidase in buffered protein solution; contains 0.02% thimerosal and 0.002% gentamicin sulfate as preservatives; contains FD&C red # 40 as coloring agent.
3. 1 bottle, 12.0 mL, SUBSTRATECHROMOGEN Buffered hydrogen peroxide and 3,3'-,5,5'- tetramethylbenzidine (TMB) solution.
4. 1 bottle, 12.0 mL, STOP SOLUTION 1 N H2SO4.
5. 1 vial, 1.0 mL, 0 ng/mL hGH CALIBRATOR/SAMPLE DILUENT: Bovine serum (Lyophilized); contains 0.02% thimerosal and 0.002% gentamicin sulfate as preservatives.
6. 5 vials, 1.0 mL, hGH CALIBRATORS: hGH in bovine serum (Lyophilized); contains 0.02% thimerosal and 0.002% gentamicin sulfate as preservatives; 2.5, 5, 10, 25, and 50 ng/mL.
Other Materials and Solutions Required
Additional Required Materials
Disposable tip precision pipets - 0.05, 0.1, 0.2 and 1.0 mL
microtiter plate reader
Distilled or deionized water
Precautions

Patient sample may contain pathogens: treat all samples as potentially infectious.
Reagents contain thimerosal; avoid contact with skin.
Avoid contact with SUBSTRATE-CHROMOGEN (tetramethyl-benzidine) solution. It is harmful if inhaled or absorbed through skin (may cause irritation).
CAUTION: Source material used to prepare Calibrators was derived from human material. The material was tested using FDA-approved methods and found non-reactive for Hepatitis B Surface Antigen (HBsAg) by ELISA and non-reactive for HIV by ELISA. No known test method can offer total assurance that infectious agents are absent.

HANDLE THESE REAGENTS AS IF THEY ARE POTENTIALLY INFECTIOUS.
Information on handling human serum is provided in the CDC/NIH manual "Bio-safety in Microbiological and Biomedical Laboratories" (1984).

Plate Preparation
Assay Procedure

1. Place sufficient COATED WELLS in a holder to run CALIBRATORS, Quality Control Sera and patient samples in duplicate. Limit run size to the number of samples that can be pipetted in 10 minutes.
2. Pipet 50.0 μL of the CALIBRATORS, Controls or Patient Sample to the corresponding COATED WELL.
3. Pipet or dispense 100 μL of the ENZYME ANTIBODY CONJUGATE solution to all the wells and mix gently.
4. Incubate at room temperature (18°- 30°C) for 45 minutes ± 5 minutes.
5. Decant or aspirate and discard liquid contents of all wells. SLAP the inverted wells on a clean piece of absorbent paper. Remove ALL OF THE LIQUID from the wells.
6. Fill each well with deionized or distilled water. Fill the wells to overflowing, you cannot cause any carryover between the wells. Decant or aspirate liquid contents of all wells. SLAP the inverted wells on a fresh clean piece of absorbent paper. Remove ALL OF THE LIQUID from the wells.
WARNING: WASHING THE WELLS IS OF CRITICAL IMPORTANCE. Fill the wells to overflowing, you CANNOT cause any carryover between wells. You CANNOT over wash the wells. Completely decant or aspirate all of the liquid out of the wells. SLAP the inverted wells on a FRESH clean piece of absorbent paper AFTER EACH WASH. YOU CANNOT SLAP TOO HARD, REMOVE ALL OF THE LIQUID FROM THE WELLS.
7. Repeat step 6 three more times (for a total of 4 washes).
8. Decant or aspirate liquid contents of all wells. SLAP the inverted wells on a fresh clean piece of absorbent paper. Remove ALL OF THE LIQUID from the wells.
9. Pipet or dispense 100 μL (0.1 mL) of SUBSTRATECHROMOGEN solution into each well.
10. Mix thoroughly and incubate 30 minutes at room temperature (18°- 30°C).
11. Pipet or dispense 100 μL (0.1 mL) of 1 N H2SO4 into each well and mix thoroughly.
12. Read the absorbance of each well at 450 ± 20 nm against water.

Background

Growth hormone (GH) is a peptide hormone that stimulates growth and cell reproduction. It is synthesized, stored, and secreted by the somatotroph cells within the lateral wings of the anterior pituitary gland. Effects of growth hormone on the tissues can generally be described as anabolic (building up). GH has several biological actions in the immune system: enhancing thymopoiesis and T cell development, modulating cytokine production, enhancing B cell development and antibody production, priming neutrophils and monocytes for superoxide anion secretion, enhancing neutrophil adhesion and monocyte migration and anti-apoptotic action. 1

References & Citations

1. Daughaday, W. H. et al. (1985) Williams Textbook of Endocrinology, Philadelphia, W. B. Saunders Co., P 568
2. Daughaday, W. H. et al. (1978) Hosp. Pract. 13: 75
3. Van Wyk, J. J. et al. (1978) Hosp. Pract. 13: 57
4. Primus, F. J. et al. Clin. Chem. 34 :261
5. Hansen H. J. et al. (1989) Clin. Chem. 35:146
6. Schroff, R. J. et al. (1985) Cancer Res. 42: 879
7. Davis, J. S. (1979) Rheumatology and Immunology Cohen, A.S. (ed.) New York: Grune and Stratton, 77
8. Highton, J. et al. (1984) J. Immunol. Meth. 68:185
9. Maiolini, R. et al. (1975) J. Immunol. Meth. 8:223
  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.