The MICRO-ELISA GROWTH HORMONE test is a solid phase sandwich-type enzyme immunoassay (ELISA) Diagnostic Kit for the In vitro quantitative determination of human growth hormone (hGH) concentration in human serum.
Components in Each 96-Test Micro-Elisa hGH Diagnostic Kit
1. 96 wells, hGH ANTIBODY COATED WELLS: Coated with anti-hGH (rabbit polyclonal); contained in a pack with silica gel desiccant.
2. 1 bottle, 12.0 mL, hGH ENZYME ANTIBODY CONJUGATE: anti-hGH (mouse monoclonal) labeled with horseradish peroxidase in buffered protein solution; contains 0.02% thimerosal and 0.002% gentamicin sulfate as preservatives; contains FD&C red # 40 as coloring agent.
3. 1 bottle, 12.0 mL, SUBSTRATECHROMOGEN Buffered hydrogen peroxide and 3,3'-,5,5'- tetramethylbenzidine (TMB) solution.
4. 1 bottle, 12.0 mL, STOP SOLUTION 1 N H2SO4.
5. 1 vial, 1.0 mL, 0 ng/mL hGH CALIBRATOR/SAMPLE DILUENT: Bovine serum (Lyophilized); contains 0.02% thimerosal and 0.002% gentamicin sulfate as preservatives.
6. 5 vials, 1.0 mL, hGH CALIBRATORS: hGH in bovine serum (Lyophilized); contains 0.02% thimerosal and 0.002% gentamicin sulfate as preservatives; 2.5, 5, 10, 25, and 50 ng/mL.
Other Materials and Solutions Required
Additional Required Materials
Disposable tip precision pipets - 0.05, 0.1, 0.2 and 1.0 mL
microtiter plate reader
Distilled or deionized water
Patient sample may contain pathogens: treat all samples as potentially infectious.
HANDLE THESE REAGENTS AS IF THEY ARE POTENTIALLY INFECTIOUS.
1. Place sufficient COATED WELLS in a holder to run CALIBRATORS, Quality Control Sera and patient samples in duplicate. Limit run size to the number of samples that can be pipetted in 10 minutes.
Growth hormone (GH) is a peptide hormone that stimulates growth and cell reproduction. It is synthesized, stored, and secreted by the somatotroph cells within the lateral wings of the anterior pituitary gland. Effects of growth hormone on the tissues can generally be described as anabolic (building up). GH has several biological actions in the immune system: enhancing thymopoiesis and T cell development, modulating cytokine production, enhancing B cell development and antibody production, priming neutrophils and monocytes for superoxide anion secretion, enhancing neutrophil adhesion and monocyte migration and anti-apoptotic action. 1
References & Citations
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8. Highton, J. et al. (1984) J. Immunol. Meth. 68:185
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