RAW 264.7 Whole Cell Lysate

RAW 264.7 (Abelson murine leukemia virus-induced tumor)

BALB/c Mouse (adult, Male) ascites, Abelson murine leukemia virus transformed; macrophage, Abelson murine leukemia virus-induced tumor

DMEM & 10% fetal bovine serum

RAW 264.7 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The RAW 264.7 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol.

One year from date of receipt

This whole cell lysate is stable for three months at -20°C or at –70°C for up to one year.

This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.