Recombinant Human Adiponectin

Pricing & Details

Product. No.A321
Alternate Names
gACRP-30, ADIPOQ, ACDC, ACRP30, APM-1, APM1, GBP28
Expression Host
E. coli
Reactivity Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
A321-25 µg
25 µg
$195.00
In stock
Max:
Min: 1
Step: 1
A321-100 µg
100 µg
$445.00
In stock
Max:
Min: 1
Step: 1
A321-500 µg
500 µg
$1,695.00
In stock
Max:
Min: 1
Step: 1
A321-1 mg
1 mg
$2,795.00
In stock
Max:
Min: 1
Step: 1

Background

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease. Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors. Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes. The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities.

Protein Details

Purity
>90% by SDS-PAGE and HPLC
Endotoxin Level
<0.125 EU/µg as determined by the LAL method
Biological Activity
ACRP-30 is fully biologically active when compared to standard. Activity is determined by the ability to inhibit the proliferation of mouse M1 cells. The expected ED50 for this effect is 1.0 – 2.5 μg/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human ACRP30 is Mr 16.7 kDa.
Formulation
Lyophilized from 10 mM Tris, pH 8.0 + 0.75 mM DTT at a concentration of 1 mg/ml.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Xu, A. et al. (2003) J. Clin. Invest. 112:91 2. Wang, Y. et al. (2002) J. Biol. Chem. 277:19521 3. Yamauchi, T. et al. (2002) Nat. Med. 8:1288 4. Berg, A. H. et al. (2001) Nat. Med. 7:947
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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