Anti-Human/Mouse/Rat p70 Ribosomal Protein S6 Kinase
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Antibody DetailsProduct DetailsReactive Species Human/Mouse/Rat Host Species Rabbit Immunogen Purified Recombinant Human/Mouse/Rat p70 S6K (>98%) Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831596 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing p70 S6K. A suggested method would be to stain cells expressing p70 S6K with 2.5 µg/106 cells in a total staining volume of 200 µl. Western Blotting: To detect Human/Mouse/Rat p70 S6K this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R1190 and a chromogenic substrate such as PN:T343. The detection limit for Human/Mouse/Rat p70 S6K is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Simple Western Blot: Recommended concentration of 2 µg/mL. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/mL. The recommended secondary antibody for IHC is PN:R1190. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Anti-Human/Mouse/Rat p70 Ribosomal Protein S6 Kinase recognizes Human/Mouse/Rat p70 Ribosomal Protein S6 Kinase. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Antigen DetailsNCBI Gene Bank ID UniProt.org Technical ProtocolsCyTOF®  IHC FFPE  |
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