Leinco Technologies provides industry-leading in vivo grade antibodies specifically designed for use in preclinical mouse model studies and other in vivo biological research applications. These high-performance antibodies are essential tools for exploring cell signaling pathways, immune response mechanisms, tumor microenvironments, and therapeutic efficacy in animal models—particularly in murine systems commonly used for immunotherapy checkpoint blockade oncology, immunology, and infectious disease research.
Our in vivo antibodies are rigorously purified to meet the highest quality standards, featuring:
- Ultra-low endotoxin levels
- No detectable protein aggregates or leachable Protein A
- Pathogen-free certification (via IDEXX Impact I PCR mouse pathogen profile)
- Highest Industry Purity Level Specifications
All antibodies are manufactured at our ISO 9001:2015 and ISO 13485:2016 certified facility in St. Louis, Missouri USA, ensuring consistency, reproducibility, and safety for animal injection and in vivo functional studies.
In vivo GOLD™ vs. in vivo PLATINUM™ Antibodies
Leinco offers two premium product lines tailored for in vivo use:
- In vivo GOLD™ Antibodies: High-purity reagents ideal for basic in vivo applications and mechanistic studies.
- In vivo PLATINUM™ Antibodies: Our top-tier antibodies, optimized for preclinical use, are verified to be pathogen-free, with low endotoxin levels and higher purity for sensitive in vivo research environments.
GOLD™ vs. PLATINUM™ in vivo Antibody Quality Standards
| Specifications | in vivo GOLD Antibodies | in vivo PLATINUM Antibodies |
|---|---|---|
| Binding validation determined by WB, FC or ELISA | Yes | Yes |
| Endotoxin level determined by the LAL method | ≤ 1.0 EU/mg | ≤ 0.5 EU/mg |
| Antibody aggregation screening by analytical SEC | ≥ 95% monomer | ≥ 98% monomer |
| Purity by SDS PAGE | ≥ 95% | ≥ 98% |
| Formulation for in vivo use | No preservatives No stabilizers No carrier protein Sterile PBS pH 7.2 – No K+ or Ca2+ Concentration: > 5mg/mL | No preservatives No stabilizers No carrier protein Sterile PBS pH 7.2 – No K+ or Ca2+ Concentration: > 5mg/mL |
| Murine pathogen screening | Not applicable, see PLATINUM functional grade antibodies | Pathogen tested (IMPACT1) (see table) |
| Applications | In vivo functional studies and may be used for WB, FC, IF or IHC | |
| Product preparation | Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. | |
| Storage and handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 1 month, 2 to 8 °C under sterile conditions, as supplied. • 12 months, -70 °C under sterile conditions. | |
IDEXX IMPACT I (PCR-based) Mouse Pathogen Profile for Purified In vivo PLATINUM™ Grade Antibodies
| Mycoplasma sp. | Mouse adenovirus 1 (MAV1) | Lactate dehydrogenase-elevating virus (LDEV) |
| Mycoplasma pulmonis | Mouse adenovirus 2 (MAV2) | Mouse kidney parvovirus (MKPV) |
| Sendai virus | Murine norovirus (MNV) | Hantaan Virus |
| Mouse hepatitis virus (MHV) | Reovirus 3 (REO3) | Mouse cytomegalovirus (mCMV) |
| Pneumonia virus of mice (PVM) | Mouse rotavirus (EDIM) | K virus |
| Minute virus of mice (MVM) | Ectromelia virus | Mouse thymic virus (MTV) |
| Mouse parvovirus (MPV) | Polyomavirus | Corynebacterium bovis |
| Theiler’s murine encephalomyelitis (TMEV) | Lymphocytic choriomeningitis virus (LCMV) | Corynebacterium sp. |
Flexible Formats for Diverse Research Needs
Our in vivo antibodies are validated for use in:
- Mouse model studies
- Flow cytometry (FACS)
- In vivo blocking or depleting experiments
- Immunohistochemistry (IHC)
- Spatial biology and tissue imaging platforms
Available in standard pack sizes: 1 mg, 5 mg, 25 mg, 50 mg, and 100 mg, with custom concentrations, bulk quantities, and custom packaging available to support the specific needs of your in vivo research program.
