In Vivo Functional Grade Antibodies

Functional Antibodies For Depletion, Activation, Neutralization, or Blocking

With proprietary methods for manufacturing and purifying in vivo functional grade antibodies, Leinco Technologies takes pride in offering these validated antibodies with the highest purity standards in the industry. The in vivo PLATINUM™ functional antibodies have the most stringent quality specifications for purity, endotoxins, aggregates and leachable protein A needed for cell culture and in vivo research. In vivo PLATINUM™ antibodies are also pathogen free and tested by the IDEXX Impact I PCR mouse pathogen profile.

Each antibody is available in custom concentrations and package sizes.

In vivo GOLD™ vs. In vivo PLATINUM™ Quality Standards

Specifications Purified in vivo GOLD functional grade mAbs Purified in vivo PLATINUM functional grade mAbs
Binding validation determined by
WB, FC or ELISA
Yes Yes
Endotoxin level determined by
the LAL method
≤ 1.0 EU/mg ≤ 0.5 EU/mg
Antibody aggregation screening by analytical SEC ≥95% monomer ≥98% monomer
Purity by SDS PAGE ≥95% ≥98%
Formulation for in vivo use
No preservatives
No stabilizers
Carrier protein free
Sterile PBS pH 7.2 – No K+ or Ca2+
Concentration: >5mg/mL
Ultra-low levels of leached Protein A
No preservatives
No stabilizers
Carrier protein free
Sterile PBS pH 7.2 – No K+ or Ca2+
Concentration: >5mg/mL
Murine pathogen screening Not applicable, see PLATINUM functional grade antibodies Pathogen tested (IMPACT1) (see table)
Applications In vivo functional studies and may be used for WB, FC, IF or IHC
Product preparation Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and handling Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 1 month, 2 to 8 °C under sterile conditions, as supplied.
• 12 months, -70 °C under sterile conditions.

IDEXX IMPACT I (PCR-based) Mouse Pathogen Profile for Purified In vivo PLATINUM™ Grade Antibodies

Mycoplasma sp. Mouse adenovirus 1 (MAV1) Lactate dehydrogenase-elevating virus (LDEV)
Mycoplasma pulmonis Mouse adenovirus 2 (MAV2) Mouse kidney parvovirus (MKPV)
Sendai virus Murine norovirus (MNV) Hantaan Virus
Mouse hepatitis virus (MHV) Reovirus 3 (REO3) Mouse cytomegalovirus (mCMV)
Pneumonia virus of mice (PVM) Mouse rotavirus (EDIM) K virus
Minute virus of mice (MVM) Ectromelia virus Mouse thymic virus (MTV)
Mouse parvovirus (MPV) Polyomavirus Corynebacterium bovis
Theiler’s murine encephalomyelitis (TMEV) Lymphocytic choriomeningitis virus (LCMV) Corynebacterium sp.

Bioanalytical Data

percent aggregates graph
Determination of Purity SDS Page graph

Explore the following In vivo antibody categories: