Functional Antibodies For Depletion, Activation, Neutralization, or Blocking
With proprietary methods for manufacturing and purifying in vivo functional grade antibodies, Leinco Technologies takes pride in offering these validated antibodies with the highest purity standards in the industry. The in vivo PLATINUM™ functional antibodies have the most stringent quality specifications for purity, endotoxins, aggregates and leachable protein A needed for cell culture and in vivo research. In vivo PLATINUM™ antibodies are also pathogen free and tested by the IDEXX Impact I PCR mouse pathogen profile.
Each antibody is available in custom concentrations and package sizes.
In vivo GOLD™ vs. In vivo PLATINUM™ Quality Standards
| Specifications | Purified in vivo GOLD functional grade mAbs | Purified in vivo PLATINUM functional grade mAbs |
|---|---|---|
| Binding validation determined by WB, FC or ELISA |
Yes | Yes |
| Endotoxin level determined by the LAL method |
≤ 1.0 EU/mg | ≤ 0.5 EU/mg |
| Antibody aggregation screening by analytical SEC | ≥95% monomer | ≥98% monomer |
| Purity by SDS PAGE | ≥95% | ≥98% |
| Formulation for in vivo use | No preservatives No stabilizers Carrier protein free Sterile PBS pH 7.2 – No K+ or Ca2+ Concentration: >5mg/mL |
Ultra-low levels of leached Protein A No preservatives No stabilizers Carrier protein free Sterile PBS pH 7.2 – No K+ or Ca2+ Concentration: >5mg/mL |
| Murine pathogen screening | Not applicable, see PLATINUM functional grade antibodies | Pathogen tested (IMPACT1) (see table) |
| Applications | In vivo functional studies and may be used for WB, FC, IF or IHC | |
| Product preparation | Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. | |
| Storage and handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 1 month, 2 to 8 °C under sterile conditions, as supplied. • 12 months, -70 °C under sterile conditions. |
|
IDEXX IMPACT I (PCR-based) Mouse Pathogen Profile for Purified In vivo PLATINUM™ Grade Antibodies
| Mycoplasma sp. | Mouse adenovirus 1 (MAV1) | Lactate dehydrogenase-elevating virus (LDEV) |
| Mycoplasma pulmonis | Mouse adenovirus 2 (MAV2) | Mouse kidney parvovirus (MKPV) |
| Sendai virus | Murine norovirus (MNV) | Hantaan Virus |
| Mouse hepatitis virus (MHV) | Reovirus 3 (REO3) | Mouse cytomegalovirus (mCMV) |
| Pneumonia virus of mice (PVM) | Mouse rotavirus (EDIM) | K virus |
| Minute virus of mice (MVM) | Ectromelia virus | Mouse thymic virus (MTV) |
| Mouse parvovirus (MPV) | Polyomavirus | Corynebacterium bovis |
| Theiler’s murine encephalomyelitis (TMEV) | Lymphocytic choriomeningitis virus (LCMV) | Corynebacterium sp. |
Bioanalytical Data
