Rabbit Monoclonal Antibody Development

Accelerated Antibody Development: Streamlined Workflow, High Throughput, Rapid Turnaround, and Customizable Genetic Engineering Ensuring Optimal Performance & Reproducibility

mAb Development Using Single B Cell Sequencing

Leinco uniquely combines proprietary methods for B-cell stimulation and cloning, high-throughput analysis, sequencing, and recombinant expression to overcome the limitations of traditional hybridoma fusion methods.

By sequencing IgG genes directly from activated clonal B-cell populations, we eliminate time-consuming hybridoma fusion and subcloning steps, for a streamlined approach with rapid turnaround times. 

The sequenced epitope recognition site can be cloned into the backbone of choice and genetically engineered as needed for optimal performance in your application.

With our rapid development workflow and cutting-edge platform, we can produce the antibodies you need in as little as 11 weeks.

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Key Benefits

High Affinity and Specificity

Rabbit monoclonal antibodies offer superior tissue penetration, high specificity, and reduced immunogenicity, making them ideal for various applications including IHC, lateral flow assays, and therapeutics.

High-Throughput Kinetic Profiling

Rapid screening of large libraries identifies the most promising candidates for sequencing while also providing high-quality binding data, accelerating drug discovery and diagnostics.

Rapid, Scalable Process

Standardized production processes enable efficient small-scale and mass production to meet any demand.

Well-Defined Sequence Suitable for Genetic Engineering

Recombinant antibodies allow for precision changes including isotype switching, Fc mutations to limit antibody-mediated cellular effects, and other design properties to make the antibodies suitable for various applications.

Exceptional Antibody Performance

Rabbits possess a highly diverse antibody repertoire, capable of recognizing a broad array of antigens with high specificity and affinity.

Enhanced repeatability with exceptional Lot-to-Lot Consistency

Recombinant antibodies ensure consistent production through standardized genetic processes, minimizing batch-to-batch variability and ensuring reproducibility critical for reliable research and therapeutic applications.

How it Works

Our recombinant rabbit mAb development process starts with the identification or design of the best immunogen and screening antigen for your project. Rabbits are then immunized to induce an immune response. We use antigen panning to isolate antibody-expressing B cells, which are then cloned and cultured in 96-well format using proprietary serum-free B cell stimulation media. B cells producing antibodies against the target antigen are characterized by high-throughput kinetic profiling to identify the most promising candidates for sequencing. The amplified antibody genes encoding the variable heavy and light chain regions are cloned into expression vectors containing the elements necessary for expression in a suitable host system (e.g., HEK or CHO cells), and cultured under conditions that promote antibody expression. Finally, the expressed antibodies are purified and characterized to confirm their specificity, affinity, and functionality using a variety of techniques such as ELISA, Western blotting, flow cytometry and functional assays appropriate for the intended application.

Phase I

Identification/Design of the best immunogen for immunization and screening antigen.

Up to 1 Week

Phase II

Development and Manufacture of immunogen and screening antigen(s) may not be the same Usually produced recombinantly to > 95% purity.

If purchased from a third party and in stock 1 week.

If developed and manufactured by Leinco ~ 4 – 8 weeks.

Phase III

Immunization of up to three New Zealand White rabbits A test bleed from each rabbit will be taken and analyzed by ELISA using the antigen to determine if the rabbits are ready to rest and perform a final boost.

6 – 10 Weeks.

Phase IV

A whole blood sample is taken from an immunized rabbit and antigen specific memory B-cells are isolated and plated into multiple 96 well culture plates containing Leinco’s proprietary serum free B-cell stimulation media to allow culture.

1 – 2 Weeks

Phase V

Each well from the 96 well plates are screened for kinetics with the antigen and this data is presented to clients for potential sequencing.

1 Week

Phase VI

Sequencing of B-cells form 96 wells plates.

2 – 3 Weeks

Phase VII

Leinco will perform gene synthesis, cloning, expression (100 ml) and purification from the sequenced clone.

1 – 2 Weeks

Why Leinco is the Solution

With over 30 years of experience, Leinco excels in antibody development and production, offering tailored solutions for both small and large-scale needs. Our client-centered approach fosters collaboration, ensuring project goals are met. Leveraging proprietary serum-free B-cell stimulation media, we optimize cell expansion for consistent, high-yield antibody production. With stringent quality control measures, Leinco guarantees reliability and innovation for all your antibody requirements.

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What to Expect

Guaranteed deliverables with competitive pricing! Count on Leinco for meticulous attention during rabbit monoclonal antibody development. From consultation to delivery, we craft tailored solutions that meet your unique specifications. Our experts employ advanced techniques for antigen design, immunization, and screening, ensuring high-affinity antibodies. Expect comprehensive support, transparent communication, and efficient project management, leading to the timely delivery of optimized antibodies in whatever quantity you require. Trust Leinco as your reliable partner for precise, cost-effective rabbit monoclonal antibody solutions.

Antibody Production

Large or small scale production of monoclonal antibodies in dedicated class 10k clean rooms from an ISO 9001:2015 and ISO 13485:2016 certified company.

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Conjugations for In Vitro Diagnostics (IVD)

Enhance diagnostic accuracy with our precision antibody conjugation, elevate your in vitro assays with reliable detection and unparalleled sensitivity, and empower your diagnostics with our expertly crafted conjugates.

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Immunoassay Development

Our scientists have exceptional experience in both creating and optimizing your assay. Avoid common pitfalls by letting us manage your assay development.

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