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Anti-PD-1 Antibodies In vivo

Programmed cell death protein 1, commonly abbreviated as PD-1, is an inhibitory receptor expressed on some tumor cells, which reduces T-cell activity. Monoclonal Anti-PD-1 antibodies block the PD-1 receptor so the T cells are no longer inhibited, activating the immune response against the tumor.

Leinco Technologies offers four different anti-PD-1 monoclonal antibodies: 29F.1A12, RMP1-14, J43, and 5C4-B8 (Nivolumab). All PD-1 clones are used to block PD-1 signaling in vivo in mouse tumor models and other mouse models. The available clones all have extensive publication records to support them. Leinco’s anti-mouse PD-1 antibodies are available in either In vivo GOLD™ and In vivo PLATINUM™ formats. Learn More

Which PD-1 Clone Is Best For My Research?

While all of Leinco’s PD-1 antibodies bind to PD-1 and block the signaling in vivo., here may be one clone that is better suited to your specific mouse model and research needs. Each antibody was created by different researchers at different times, however, they were all created using standard hybridoma technology. 

Clone: RMP1-14

Isotype: Rat IgG2a, k

Original Citation: Was first published in 2005 by Takanori Kanai and Hideo Yagit, et al.

Leinco in the Literature: RMP1-14

Immunogen: Syrian hamster BKH cells transfected with mouse PD-1 cDNA.

Purity: >95% Purity by SDS Page and HPLC

Preparation: Contains no preservatives or carrier proteins

Applications: According to the literature, clone RMP1-14, is a good Fluorescence-Activated Cell Sorting (FACS) mab and a good blocker of PD-L1 or PD-L2 binding to PD-1; Effective in immunotherapy experiments. NOTE: We would not suggest using this clone to stain cells from a 29F.1A12-treated animal or vice versa.

Product
Clone
Applications
Prod No.
Package Size
RMP1-14
FC
IHC FFPE
WB
C2857
0.5 mg
RMP1-14
B
FA
WB
P362
1.0 mg
100 mg
25 mg
5.0 mg
50 mg
RMP1-14
B
FA
WB
P372
1.0 mg
100 mg
25 mg
5.0 mg
50 mg

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Clone: 29F.1A12

Isotype: Rat IgG2a

Original Citation: Was first published in 2003 by Spencer C. Liang and Gordon Freeman et al.

Immunogen: Recombinant mouse PD-1-Ig fusion protein.

Purity: >95% Purity by SDS Page and HPLC

Preparation: Contains no preservatives or carrier proteins

Applications: According to the literature, 29F.1A12, PD-1 mAb (rat IgG2a isotype), is a good FACS mab and a good blocker of PD-L1 or PD-L2 binding to PD-1; Effective in immunotherapy experiments. This clone can also work very well in western blots.

Product
Clone
Applications
Prod No.
Package Size
29F.1A12
FC
IHC FFPE
WB
P383
0.5 mg
29F.1A12
B
CODEX®
CyTOF®
FC
IHC FF
WB
P377
1.0 mg
100 mg
25 mg
5.0 mg
50 mg
29F.1A12
B
CODEX®
CyTOF®
FC
IHC FF
WB
P378
1.0 mg
100 mg
25 mg
5.0 mg
50 mg

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Clone: 5C4.B8

Isotype: Human IgG1, k

Original Citation: Was first published in 2014 by Changyu Wang, et al.

Immunogen: Used to create this antibody is reported as 1) recombinant human PD-1-Fc protein consisting of the extracellular domain of PD-1 (amino acids 1–167) and the Fc portion of human IgG1, 2) Chinese hamster ovary (CHO) cells expressing human PD-1. Transgenic mice with comprising germline configuration human immunoglobulin miniloci in an endogenous IgH and Igκ knockout background were used to develop this antibody.

Purity: >95% Purity by SDS Page and HPLC

Preparation: Contains no preservatives or carrier proteins

Product
Clone
Applications
Prod No.
Package Size
5C4.B8
B
FA
FC
IHC
LT1200
0.2 mg
1.0 mg
25 µg
Bulk
5C4.B8
B
FA
FC
IHC
LT1205
0.2 mg
1.0 mg
25 µg
Bulk

Clone: J43

Isotype: Armenian hamster IgG

Original Publication: Was first published in 1996 by Yasutoshi Agata et al.

Immunogen: Syrian hamster BKH cells transfected with mouse PD-1 cDNA.

Purity: >95% Purity by SDS Page and HPLC

Preparation: Contains no preservatives or carrier proteins

Applications: Researchers have observed and reported that clone J43 to have effector function in vivo as tumors were observed to grow faster during J43 therapy. See Publication Table 1. Additionally, J43 may also be neutralized more rapidly than RMP1-14.  

Product
Clone
Applications
Prod No.
Package Size
J43
FC
P348
500 µg
J43
ICC
P350
0.5 mg
1.0 mg

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