Anti-PD-1 Antibodies in vivo
Programmed cell death protein 1, commonly abbreviated as PD-1, is an inhibitory receptor expressed on some tumor cells, which reduces T-cell activity. Monoclonal Anti-PD-1 antibodies block the PD-1 receptor so the T cells are no longer inhibited, activating the immune response against the tumor.
Leinco Technologies offers three (3) unique anti-PD-1 monoclonal antibodies: 29F.1A12, RMP1-14, 384-35 (fully murinized). All PD-1 clones are used to block PD-1 signaling in vivo in mouse tumor models. Clone 384-35 is part of a new class of mouse anti-mouse antibodies that significantly increases survival past 100 days compared to RMP1-14 and shows tumor volume more than 2.5 fold smaller compared to RMP1-14. The available clones all have extensive publication records to support them. Leinco’s anti-mouse PD-1 antibodies are available in both in vivo GOLD™ and in vivo PLATINUM™ formats.
See below for more information on our available selections of PD-1 antibodies and how to appropriately select one for your use case. Don’t see an antibody for your experiment? Check out our monoclonal antibody manufacturing services and let us know what you need. We also provide antibody conjugation services if you require specific tagging for your research and diagnostic applications.
Which PD-1 Clone Is Best For My Research?
While all of Leinco’s PD-1 antibodies bind to PD-1 and block the signaling in vivo, here may be one clone that is better suited to your specific mouse model and research needs. Each antibody was created by different researchers at different times; however, they were all created using standard hybridoma technology.
| Clone | RMP1-14 | 384-35 | 29F.1A12 |
|---|---|---|---|
| Isotype | Rat IgG2a, k | Mouse IgG1 | Rat IgG2a |
| Original Citation | Was first published in 2005 by Takanori Kanai and Hideo Yagit, et al. | Was first published in 2003 by Spencer C. Liang and Gordon Freeman et al. | |
| Leinco in the Literature | RMP1-14 | ||
| Immunogen | Syrian hamster BKH cells transfected with mouse PD-1 cDNA. | Recombinant mouse PD-1 used in PD-1 -/- knockout mouse | Recombinant mouse PD-1-Ig fusion protein. |
| Purity | >95% Purity by SDS Page and HPLC | >95% Purity by SDS Page and HPLC | >95% Purity by SDS Page and HPLC |
| Preparation | Contains no preservatives or carrier proteins | Contains no preservatives or carrier proteins | Contains no preservatives or carrier proteins |
| Recommended Isotype Control | Rat IgG2a PLATINUM Or Rat IgG2a GOLD |
Mouse IgG1 GOLD | Rat IgG2a PLATINUM Or Rat IgG2a GOLD |
| Recommended Dilution Buffer | D230 | D230 | D230 |
| Applications | Clone RMP1-14 is a good Fluorescence-Activated Cell Sorting (FACS) mab and a good blocker of PD-L1 or PD-L2 binding to PD-1; Effective in immunotherapy experiments. NOTE: We would not suggest using this clone to stain cells from a 29F.1A12-treated animal or vice versa. | Clone 384-35 has been used for in vivo functional assay for blocking the binding of PD-L1 to PD-1. | 29F.1A12, PD-1 mAb (rat IgG2a isotype), is a good FACS mab and a good blocker of PD-L1 or PD-L2 binding to PD-1; Effective in immunotherapy experiments. This clone can also work very well in western blots. |
Categories
Filters
Product | Clone | Applications | Prod No. | Package Size |
|---|---|---|---|---|
RMP1-30 | Depletion ⋅ FC ⋅ in vivo | C3442 | 0.5 mg ⋅ 1.0 mg ⋅ 100 mg ⋅ 25 mg ⋅ 5 mg ⋅ 50 mg |
Product | Clone | Applications | Prod No. | Package Size |
|---|---|---|---|---|
384-35 | B ⋅ FA ⋅ in vivo | P710 | 1.0 mg ⋅ 100 mg ⋅ 25 mg ⋅ 5.0 mg ⋅ 50 mg ⋅ Custom |
Product | Clone | Applications | Prod No. | Package Size |
|---|---|---|---|---|
29F.1A12 | B ⋅ CyTOF® ⋅ FC ⋅ IHC FF ⋅ in vivo ⋅ PhenoCycler® ⋅ WB | P377 | 1.0 mg ⋅ 100 mg ⋅ 25 mg ⋅ 5.0 mg ⋅ 50 mg | |
29F.1A12 | B ⋅ CyTOF® ⋅ FC ⋅ IHC FF ⋅ in vivo ⋅ PhenoCycler® ⋅ WB | P378 | 1.0 mg ⋅ 100 mg ⋅ 25 mg ⋅ 5.0 mg ⋅ 50 mg |
