Anti-PD-1 Antibodies in vivo
Programmed cell death protein 1, commonly abbreviated as PD-1, is an inhibitory receptor expressed on some tumor cells, which reduces T-cell activity. Monoclonal Anti-PD-1 antibodies block the PD-1 receptor so the T cells are no longer inhibited, activating the immune response against the tumor.
Leinco Technologies offers three (3) unique anti-PD-1 monoclonal antibodies: 29F.1A12, RMP1-14, 384-35 (fully murinized). All PD-1 clones are used to block PD-1 signaling in vivo in mouse tumor models. Clone 384-35 is part of a new class of mouse anti-mouse antibodies that significantly increases survival past 100 days compared to RMP1-14 and shows tumor volume more than 2.5 fold smaller compared to RMP1-14. The available clones all have extensive publication records to support them. Leinco’s anti-mouse PD-1 antibodies are available in both in vivo GOLD™ and in vivo PLATINUM™ formats.
Which PD-1 Clone Is Best For My Research?
While all of Leinco’s PD-1 antibodies bind to PD-1 and block the signaling in vivo., here may be one clone that is better suited to your specific mouse model and research needs. Each antibody was created by different researchers at different times, however, they were all created using standard hybridoma technology.
Clone: RMP1-14
Isotype: Rat IgG2a, k
Original Citation: Was first published in 2005 by Takanori Kanai and Hideo Yagit, et al.
Leinco in the Literature: RMP1-14
Immunogen: Syrian hamster BKH cells transfected with mouse PD-1 cDNA.
Purity: >95% Purity by SDS Page and HPLC
Preparation: Contains no preservatives or carrier proteins
Applications: According to the literature, clone RMP1-14, is a good Fluorescence-Activated Cell Sorting (FACS) mab and a good blocker of PD-L1 or PD-L2 binding to PD-1; Effective in immunotherapy experiments. NOTE: We would not suggest using this clone to stain cells from a 29F.1A12-treated animal or vice versa.
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Clone: 384-35
Isotype: Mouse IgG1
Immunogen: Recombinant mouse PD-1 used in PD-1 -/- knockout mouse
Purity: >95% Purity by SDS Page and HPLC
Preparation: Contains no preservatives or carrier proteins
Applications: Clone 384-35 has been used for in vivo functional assay for blocking the binding of PD-L1 to PD-1.
Product | Clone | Applications | Prod No. | Package Size |
|---|---|---|---|---|
384-35 | B ⋅ FA ⋅ in vivo | P710 | 1.0 mg ⋅ 100 mg ⋅ 25 mg ⋅ 5.0 mg ⋅ 50 mg ⋅ Custom |
Clone: 29F.1A12
Isotype: Rat IgG2a
Original Citation: Was first published in 2003 by Spencer C. Liang and Gordon Freeman et al.
Immunogen: Recombinant mouse PD-1-Ig fusion protein.
Purity: >95% Purity by SDS Page and HPLC
Preparation: Contains no preservatives or carrier proteins
Applications: According to the literature, 29F.1A12, PD-1 mAb (rat IgG2a isotype), is a good FACS mab and a good blocker of PD-L1 or PD-L2 binding to PD-1; Effective in immunotherapy experiments. This clone can also work very well in western blots.
Product | Clone | Applications | Prod No. | Package Size |
|---|---|---|---|---|
29F.1A12 | FC ⋅ IHC FF ⋅ PhenoCycler® ⋅ WB | P383 | 0.5 mg | |
29F.1A12 | B ⋅ CyTOF® ⋅ FC ⋅ IHC FF ⋅ in vivo ⋅ PhenoCycler® ⋅ WB | P377 | 1.0 mg ⋅ 100 mg ⋅ 25 mg ⋅ 5.0 mg ⋅ 50 mg | |
29F.1A12 | B ⋅ CyTOF® ⋅ FC ⋅ IHC FF ⋅ in vivo ⋅ PhenoCycler® ⋅ WB | P378 | 1.0 mg ⋅ 100 mg ⋅ 25 mg ⋅ 5.0 mg ⋅ 50 mg |
