≥ 5.0 mg/ml
< 1.0 EU/mg as determined by the LAL method
≥95% monomer by analytical SEC
>95% by SDS Page
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day 2-8°C
This Mouse IgG isotype control antibody should be used at the same concentration as the primary antibody.
Applications and Recommended Usage?
Quality Tested by Leinco
FC This isotype control antibody should be used at the same concentration as the primary antibody.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
This Mouse IgG1 isotype control antibody (Anti-BTV) has been tested against selected species' cells and tissues to assure minimal cross reactivity.
References & Citations
1.) Crack, Peter J. et al. (2016) eNeuro 10.1523/ENEURO.0128-15.2016 Article Link
2.) Shin, Haina et al. (2018) J Virol. 92(7): e00038-18. PubMed
3.) Karki et al. (2021) Cell. 184:149–168 Journal Link
4.) Ortiz et al. (2019) Cancer Cell. 35:33–45 Journal Link
5.) Smyth et al. (2020) Cancer Immunol Res 8:1-12 Journal Link
6.) Swaminathan, S. et al. (2020) Nat Commun 11: 2860 Journal Link
7.) Karki, R. et al. (2020) Cell 184(1):149–168.e17 Journal Link