Multiple Upstream Production Platforms
A key factor to expedite delivery and reduce production costs is the availability of multiple scale upstream production platforms. Leinco can fulfill any production need while using either Steam In Place (SIP) stirred tank, packed beds, GE Life Science WAVE Bioreactors™ or proprietary single-use hollow fiber XP2000 systems. In cases where projects are in the proof of concept stage, In vitro antibody production can be performed in roller bottles or shake flasks.
- For B cell hybridomas (<250 milligrams) – roller bottles are a perfect choice and single-use platform.
- For milligram quantities of recombinant antibodies – shake flasks are often utilized followed by IMAC or other purification methods.
For large scale production quantities – steam in place (SIP) stirred tank bioreactors or hollow fiber systems are available in dedicated cleanroom suites for each product.
Cell Banking and Cell Line Optimization
Leinco Technologies provides cell line banking, mAb production optimization, and adaptation to specialty culture media for mAb production. Leinco has a dedicated unit at the St. Louis facility that develops stable B cell hybridoma cell lines, creates master cell banks and working cell banks for use in IVD diagnostics and biopharmaceutical manufacturing. Cell lines are sub-cloned and specific productivity rates are determined to maximize protein expression. Serological testing and dual-site storage of cell banks is available.