Custom Conjugation Services for Antibodies and Proteins
Since Leinco Technologies was founded in 1992, our scientists have developed and optimized a panel of immunochemistry procedures such as conjugation of antibodies and proteins to enzymes or fluorescent reporter molecules and digestion of antibodies into F(ab‘) or F(ab‘)2 fragments. Leinco’s custom antibody conjugation services are used for early discovery research or as components for in vitro diagnostic kits to assess disease states.
Protein chemists at Leinco Technologies employ linkage and post conjugation purification techniques that result in conjugates that have increased signal to background ratios for product applications such as flow cytometry, ELISA, Western blotting, immunohistochemistry and membrane assays. Common reporter molecules used to tag antibodies for both research and diagnostic applications are: Alkaline Phosphatase (AP), Horseradish Peroxidase (HRP), Allophycocyanin (APC), Fluorescein (FITC), R-Phycoerythrin (R-PE), Texas Red, Rhodamine, PerCP, and Dylight® 488, 549, 594, 649.
|Reporter Molecule||MW (g/mol)
(x 10 -3)
|ε (M -1cm-1)||Em. Max.
|Alkaline Phosphatase (AP)||140||280|
|Horseradish Peroxidase (HRP)||45.9||403|
Note: Depending upon excitation and emission requirements, a variety of other reporter molecules are available to be covalently linked to antibodies or proteins.
Final Fill Finish
Antibody and protein conjugates are formulated in stability buffers that increase product shelf life and optimize performance. Final products may be packaged in vials ready for assembly in commercial In vitro diagnostic kits or in bulk to aliquot into working volumes by research scientists.
Preparation of F(ab‘) or F(ab‘)2 Fragments of Antibodies
Leinco Technologies has extensive experience in enzyme digestion of antibodies into univalent F(ab‘) or F(ab‘)2 fragments for use in immunoassays, immunohistochemistry and In vivo functional assays. Antibody fragments may be adsorbed against serum proteins to remove undesired species cross-reactivity and conjugated to the required reporter molecule.