Providing High Quality Verified and Validated Conjugates Since 1992
Our scientists have been providing high-quality services for conjugation of antibodies and proteins to enzymes, fluorescent reporter molecules and oligonucleotide barcodes. These verified (by IEF) and validated conjugates are suitable for early discovery research or as components for in vitro diagnostic kits to assess disease states.
Protein chemists at Leinco Technologies employ optimized linkage and post-conjugation purification techniques producing conjugated antibodies and proteins with high signal to background ratios ideal for applications such as flow cytometry, ELISA, Western blotting, immunohistochemistry, spatial biology and membrane assays. We offer a wide variety of fluorophore reporter molecules commonly used to tag antibodies for detection in both research and diagnostic applications. We also offer custom antibody-barcode conjugation services for PhenoCycler-Fusion (CODEX)® spatial biology applications.
Key Benefits of Using Our Custom Conjugation Services
- Reliable, repeatable conjugation process
- Flexible conjugation chemistry can be used
- Free dye and/or antibody purification or removal
- Identification of optimal degree of labeling (DOL)
Available Reporter Molecules
| Reporter Molecule | MW (g/mol) (x 10 -3) |
Abs. Max. (nm) |
ε (M -1cm-1) | Em. Max. (nm) |
| Alkaline Phosphatase (AP) | 140 | 280 | ||
| Horseradish Peroxidase (HRP) | 45.9 | 403 | ||
| Allophycocyanin (APC) | 104 | 650 | 700,000 | 660 |
| Fluorescein (FITC) | 0.473 | 495 | 74,000 | 519 |
| R-Phycoerythrin (R-PE) | 240 | 565.5 | 1,960,000 | 578 |
| Texas Red | 0.817 | 583 | 116,000 | 603 |
| Rhodamine | 0.430 | 540 | 95,000 | 565 |
| PerCP | 35 | 482 | 40,600 | 675 |
| Dylight® 488 | 1.011 | 493 | 70,000 | 518 |
| Dylight® 549 | 0.982 | 562 | 150,000 | 576 |
| Dylight® 594 | 1.078 | 593 | 80,000 | 618 |
| Dylight® 649 | 1.008 | 654 | 250,000 | 673 |
Note: Depending upon excitation and emission requirements, a variety of other reporter molecules are available to be covalently linked to antibodies or proteins. Additional information on DyLight® Fluorophores.
Final Fill Finish
Antibody and protein conjugates are formulated in stability buffers that increase product shelf life and optimize performance. Final products may be packaged in vials ready for assembly in commercial in vitro diagnostic kits or in bulk to aliquot into working volumes by research scientists.
In addition, Leinco offers custom product formulations such as:
- BSA-free
- Azide-free
- Low endotoxin azide-free
