Decontamination of Sheath Fluid Tanks for Cell Sorters Protocol
Methods and Principles from our Scientific Staff
This user guide was developed by Leinco Technologies, the leader in sheath fluid and flow cytometry workflow solutions.
1. Add Bleach To Tank
Place enough 10% Bleach in the empty tank so that you may rotate the tank and expose all inner surfaces with the lid closed. Also coat valves on the outside.
2. Wait 1 hour
Allow to sit for at least one hour.
3. Rinse
Rinse tanks with sterile DI water at least 3 times including valves to remove bleach.
4. Spray Alcohol
Spray 95% ethanol on valves and inside of tank including aspiration tube within the sheath tank.
5. Drain and Air Dry
Drain and allow to air dry completely over night in a sterile hood.
6. Use Sterile Concentrate
Fill sheath tank with sterile concentrate (Leinco Prod. No.: S632 or P349) and sterile DI water mixture per specification.
