Recombinant Human Dkk-1

tlnsvlnsn aiknlppplg gaaghpgsav saapgilypg gnkyqtidny qpypcaedee cgtdeycasp trggdagvqi clacrkrrkr cmrhamccpg nyckngicvs sdqnhfrgei eetitesfgn dhstldgysr rttlsskmyh tkgqegsvcl rssdcasglc carhfwskic kpvlkegqvc tkhrrkgshg leifqrcycg eglscriqkd hhqasnssrl htcqrh

Thr32

Lyophilized

The predicted molecular weight of Recombinant Human Dkk-1 is Mr 25.8 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 33-40 kDa.

25.8

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 10⁵ continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.