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Anti-Human CD30 Ligand (CD30L)
Purified No Carrier Protein
(TNFSF8, CD153)
Monoclonal Antibody
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Prod. No.:C1379
Clone:116614
Isotype:Mouse IgG2b
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CD30 Ligand (Clone 116614) recognizes an epitope on Human CD30 Ligand. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody showed no cross-reactivity with rhCD27 Ligand, rmCD30 Ligand or rhCD40 Ligand.

Background:
The CD30 ligand is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. Recombinant CD30L has been shown to co-stimulate T cells and to act as a mitogen for Hodgkin's disease derived cell lines. The receptor for CD30L, CD30/ TNFRSF8/CD153, is a type I cytokine receptor that is highly expressed by activated T cells, Hodgkin and Reed- Sternberg (H-RS) cells, and anaplastic large cell lymphoma cells. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis.

Known Species Cross Reactivity: Ms-, Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human CD30 Ligand (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, FC, N
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human CD30 Ligand is approximately 5 ng/well.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD30 Ligand. A suggested method would be to stain cells expressing CD30 Ligand with 0.25 µg per 1-2.5 x 105 cells in a total staining volume of ≤200 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189).
Neutralization: This antibody is useful for neutralization of Human CD30 Ligand bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CD30 Ligand (at 1.0 ng/ml) is 1.0-4.0 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189)
Recombinant Human CD30 Ligand (Leinco Prod. No.: C1334)
Recombinant Mouse CD30 Ligand (Leinco Prod. No.: C1354)
Anti-Mouse CD30 Ligand (Leinco Prod. No.: C1455)
Anti-Human CD30 Ligand (TNFSF8) (Leinco Prod. No.: C1456)
Anti-Mouse CD30 Ligand (Antigen Aff. Pur.) (Leinco Prod. No.: C1476)
Anti-Human CD30 Ligand-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1488)
Anti-Mouse CD30 Ligand-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1521)
Anti-Human CD30 Ligand (Antigen Ant. Pur.) (Leinco Prod. No.: C1367)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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