Anti-Human CD30 Ligand (TNFSF8)

Pricing & Details

Product No.C1456
Clone
116615
Protein
CD30 Ligand
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TNFSF8, CD153
Isotype
Mouse
IgG2a
Applications
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1456-25 µg
25 µg
$190.00
In stock
Max:
Min: 1
Step: 1
C1456-500 µg
500 µg
$525.00
In stock
Max:
Min: 1
Step: 1
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Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
NS0-Derived Recombinant Human CD30 Ligand Extracellular Domain (Accession # P32971)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human CD30 Ligand this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CD30 Ligand is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human CD30 Ligand (Clone 116615) recognizes an epitope on Human CD30 Ligand. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
The CD30 ligand is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. Recombinant CD30L has been shown to co-stimulate T cells and to act as a mitogen for Hodgkin's disease derived cell lines. The receptor for CD30L, CD30/ TNFRSF8/CD153, is a type I cytokine receptor that is highly expressed by activated T cells, Hodgkin and Reed- Sternberg (H-RS) cells, and anaplastic large cell lymphoma cells. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis.

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Croager, EJ. et al. (1997) Biochim. Biophys. Acta. 1353:231
2. Gruss, HJ. et al. (1994) Blood 83:1521
3. Wendtner, CM. et al. (1995) Cancer Res. 55:4157
4. Fischer, M. et al. (2006) J. Clin. Invest. 116:2748
5. Blazar, BR. et al. (2004) J. Immunol. 173:2933
General Western Blot Protocol

Formats Available

Prod No.
Description
C1456
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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