Anti-Human Apoptosis Antagonizing Transcription Factor (AATF) [A239] : Leinco Technologies, excellence in early discovery research

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Rabbit Anti-Human Apoptosis Antagonizing Transcription Factor (AATF)

Purified No Carrier Protein

(Che-1, DED)

Polyclonal Antibody

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Prod. No.:	A239
Host:	Rabbit
Conc.:	0.5 mg/ml
Pkg. Size:	0.1 mg, 0.5 mg, 1 mg
Storage:	2-8°C Detailed storage instructions below.

Description

Specificity:

Rabbit Anti-Human AATF recognizes human and mouse Apoptosis Antagonizing Transcription Factor (AATF). This polyclonal antibody was purified using affinity chromatography.

Background:

AATF (apoptosis antagonizing transcription factor) was initially discovered as an interaction partner of ZIP kinase (ZIPK) (1), a member of death-associated protein (DAP) kinase family of pro-apoptotic serine/threonine kinases (2). AATF is a phosphoprotein containing an acidic region and a putative leucine zipper domain and nuclear localization signal, features which are typical of transcription factors. AATF inhibits the ZIPK-mediated pro-apoptotic pathway and may activate other anti-apoptotic pathways (3). Recently, it has also been shown to protect neural cells against oxidative damage induced by amyloid b-peptide and to inhibit aberrant production of the b-peptide by interacting with Par-4 (prostate apoptosis response-4), another pro-apoptotic leucine zipper protein that is associated with neuronal degeneration in Alzheimer’s disease (AD) (4,5), suggesting that AATF may have potential therapeutic applications in both familial and sporadic forms of AD.

Known Species Cross Reactivity: Ms+, Hu+

Immunogen

Apoptosis Antagonizing Transcription Factor Blocking Peptide (Leinco Prod. No.: A240)

Blocking Peptide

Apoptosis Antagonizing Transcription Factor Blocking Peptide (Leinco Prod. No.: A240)

Formulation

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

Storage and Stability

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Reported Applications

IHC (NBF/Par.), WB

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentraion of 10 µg/ml. The positive control tissue for IHC is human or mouse small intestine.

Western Blotting: This antibody can be used to detect Human AATF by Western blot analysis at a concentration of 0.5-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Small Intestine Tissue Lysate (Leinco Prod. No.: H1412).

Each investigator should determine their own optimal working dilution for specific applications.

Country of Origin

USA

References

1. AATF antibody can be stored at 4˚C, stable for one year. Page G, Lodige I, Kogel D et al. AATF, a novel transcription factor that interacts with Dlk/ZIP kinase and interferes with apoptosis. FEBS Lett. 1999; 462:187-191.

2. Kawai T, Matsumoto M, Takeda K, et al. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol. Cell Biol. 1998; 18:1642-51.

3. Lindfors K, Halttunen T, Huotari P, et al. Identification of novel transcription factor-like gene from human intestinal cells. Biochem. Biophys. Res. Commun. 2000; 276:660-6.

4. Xie J and Guo Q. AATF protects neural cells against oxidative damage induced by amyloid b-peptide. Neurobiol. of Dis. 2004; 16:150-7.