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Recombinant Human B-Cell Activating Factor Receptor (BAFF-R)
Purified No Carrier Protein
(BAFF Receptor BR3, TNFRSF13, BlyS Receptor 3)
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Prod. No.:B131
SourceE. coli
Pkg. Size:50 µg, 1 mg
Storage:-20°C Detailed storage instructions below.
Description
Background:
BAFF receptor (BAFF R/TNFRSF13/BlyS) is a type III transmembrane protein and member of the TNF receptor (TNFR) super family. It is highly expressed in spleen, lymph node, resting B cells, peripheral blood leukocytes and to some extent, in activated B cells and resting CD4+ cells. BAFF R plays a role in nearly all stages of differentiation of B cells from development to the maintenance of long-lived bone marrow plasma cells (1). BAFF R is one of three receptors, including TACI and BCMA, identified for BAFF. BAFF R signals by activation of the NFκB signaling pathway (2). Overexpression of the BAFF R ligand, BAFF, in mice leads to autoimmune characteristics similar to systemic lupus erythematosus (SLE) and Sjogren’s syndrome. Furthermore, high levels of BAFF have been detected in the serum of humans with SLE, rheumatoid arthritis, Sjogren’s syndrome and systemic sclerosis.

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human BAFF-R is Mr 34 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 40-50 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human BAFF Receptor is determined by its ability to block mouse BAFF induced mouse splenocyte survival. The expected ED50 for this effect is 4.0 µg/ml in the presence of 1 µg/ml of human soluble BAFF.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
rrgprslrg rdapaptpcv paecfdllvr hcvacgllrt prpkpagass paprtalqpq esvgagagea adiegrmdpk scdkthtcpp cpapellggp svflfppkpk dtlmisrtpe vtcvvvdvsh edpevkfnwy vdgvevhnak tkpreeqyns tyrvvsvltv lhqdwlngke ykckvsnkal papiektisk akgqprepqv ytlppsrdel tknqvsltcl vkgfypsdia vewesngqpe nnykttppvl dsdgsfflys kltvdksrwq qgnvfscsvm healhnhytq kslslspgk



Country of Origin
USA
References
1. Thompson, J. S. et al. (2001) Science 293:2108
2. Mackay, F. et al. (2002) Nature Rev. Immunol. 2:464
Related Products
Anti-Human BAFF-R (CT) (Leinco Prod. No.: B514)
BAFF-R (CT) Blocking Peptide (Leinco Prod. No.: B504)
Anti-Human BAFF R (Antigen Aff. Pur.) (Leinco Prod. No.: B627)
Anti-Human BAFF R-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B646)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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