Recombinant Human BAFF-R

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Pricing & Details

Product. No.B131
Alternate Names
BAFF Receptor BR3, TNFRSF13, BlyS Receptor 3
Expression Host
NS0 cells
Species
Human
Prod No.
Size
Price
Avail.
Qty
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B131-50 µg
50 µg
$349.00
In stock
Max:
Min: 1
Step: 1

Background

BAFF receptor (BAFF R/TNFRSF13/BlyS) is a type III transmembrane protein and member of the TNF receptor (TNFR) super family. It is highly expressed in spleen, lymph node, resting B cells, peripheral blood leukocytes and to some extent, in activated B cells and resting CD4+ cells. BAFF R plays a role in nearly all stages of differentiation of B cells from development to the maintenance of long-lived bone marrow plasma cells. 1 BAFF R is one of three receptors, including TACI and BCMA, identified for BAFF. BAFF R signals by activation of the NFκB signaling pathway.2 Overexpression of the BAFF R ligand, BAFF, in mice leads to autoimmune characteristics similar to systemic lupus erythematosus (SLE) and Sjogren’s syndrome. Furthermore, high levels of BAFF have been detected in the serum of humans with SLE, rheumatoid arthritis, Sjogren’s syndrome and systemic sclerosis.

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human BAFF Receptor is determined by its ability to block mouse BAFF induced mouse splenocyte survival.3,4,5 The expected ED50 for this effect is 4.0 µg/ml in the presence of 1 µg/ml of human soluble BAFF.
Fusion Protein Tag
Fc Fusion Protein
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
slrgrdapap tpcvpaecfd llvrhcvacg llrtprpkpa gasspaprta lqpqesvgag ageaadiegr mdpkscdkth tcppcpapel lggpsvflfp pkpkdtlmis rtpevtcvvv dvshedpevk fnwyvdgvev hnaktkpree qynstyrvvs vltvlhqdwl ngkeykckvs nkalpapiek tiskakgqpr epqvytlpps rdeltknqvs ltclvkgfyp sdiavewesn gqpennyktt ppvldsdgsf flyskltvdk srwqqgnvfs csvmhealhn hytqkslsls pgk
N-terminal Sequence Analysis
Ser7
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human BAFF-R is Mr 33 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 40-50 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
NCBI Gene Bank

References & Citations

1. Thompson, JS. et al. (2001) Science 293:2108
2. Mackay, F. et al. (2002) Nature Rev. Immunol. 2:464
3. Moore, PA. et al. (1999) Science 285:260
4. Gross, JA. et al. (2000) Nature 404:995
5. Schneider, P. et al. (1999) J. Exp. Med. 189:1747
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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