AIF peptide is applicable as a control peptide for blocking antibody binding in Western blotting.
Amino Acid Location
This peptide sequence is located at amino acids 593 to 606 of Human AIF protein.
This peptide is formulated in PBS pH 7.2 (0.01 M Sodium Phosphate, 0.13 M NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage and Stability
Store this peptide in working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
IHC (NBF/Par.), N, WB
Neutralization: The peptide may be used to neutralize the binding activity of Anti-Human Apoptosis Inducing Factor (CT) (AIF) (Leinco Prod. No.: A151) by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
1. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis - inducing factor. Nature 1999;397:441-6