Specificity:

Anti-Human Caspase-3 Active Subunit (p17) (clone C31.129) recognizes the human 17-20 kDa cleaved active subunit of caspase 3 and does not react with the inactive proenzymes. The antibody was developed using a synthetic peptide with residues mapping to the N-terminus of the (p17) subunit of human caspase 3. The antibody has not been tested for species cross-reactivity or cross reactivity with other caspases.

Background:

Caspase-3 is a member of the caspase family of cysteine proteases and plays a key role in mediating Fas (CD95) and tumor necrosis factor–1 (TNFR-1) induced apoptosis. Caspases can be divided into three groups: apoptotic initiators, apoptotic executioners, and inflammatory mediators.^1,2 Caspase-3 is activated during apoptotic signaling events by upstream proteases including caspase 6 and caspase 8.³ Caspase-3 is thought to be essential for chromatin margination, DNA fragmentation and nuclear collapse during apoptosis.³

Known Species Cross Reactivity: Hu+