This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Anti-Human Caspase-3 Active Subunit (p17) (clone C31.129) recognizes the human 17-20 kDa cleaved active subunit of caspase 3 and does not react with the inactive proenzymes. The antibody was developed using a synthetic peptide with residues mapping to the N-terminus of the (p17) subunit of human caspase 3. The antibody has not been tested for species cross-reactivity or cross reactivity with other caspases.
Caspase-3 is a member of the caspase family of cysteine proteases and plays a key role in mediating Fas (CD95) and tumor necrosis factor–1 (TNFR-1) induced apoptosis. Caspases can be divided into three groups: apoptotic initiators, apoptotic executioners, and inflammatory mediators.1,2 Caspase-3 is activated during apoptotic signaling events by upstream proteases including caspase 6 and caspase 8.3 Caspase-3 is thought to be essential for chromatin margination, DNA fragmentation and nuclear collapse during apoptosis.3
References & Citations
1. Adadah, BA. et al. (2000) Journal of Neurotoma 17(10): 811
2. Salvesen, GS. et al. (1999) Proc. Natl. Acad. Sci. USA 96(20): 10964
3. Slee, EA. et al. (2001) J. Biol. Chem. 276(10):7320
Products are for research use only. Not for use in diagnostic or therapeutic procedures.