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Goat Anti-Human Apolipoprotein E3 (ApoE3)
Antigen Affinity Purified
Polyclonal Antibody
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Prod. No.:A418
Host:Goat
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Human Anti-Human (ApoE3) recognizes Human ApoE3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human ApoE3 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 0.5 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.2 mg/ml.
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as . The detection sensitivity of this indirect ELISA for Human ApoE3 is approximately 2 ng/well.
Western Blotting: To detect Human ApoE3 this polyclonal antibody can be used at a concentration of 0.1 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human Apolipoprotein E3 (Leinco Prod. No.: A410).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human Apolipoprotein E3 (Leinco Prod. No.: A410)
Anti-Human ApoE3 (Antigen Aff. Pur.) (Leinco Prod. No.: A102)
Anti-Human ApoE3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: A103)
Human ApoE3 ELISA Development Kit (Leinco Prod. No.: A424)
Recombinant Human ApoE3 (Leinco Prod. No.: A218)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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