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Goat Anti-Human A Proliferation-Inducing Ligand (APRIL)
Biotin
(TNFSF13, TRDL-1α)
Polyclonal Antibody
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Prod. No.:A207
Host:Goat
Pkg. Size:50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Human A Proliferation-Inducing Ligand (APRIL) recognizes Human APRIL. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human APRIL (>98%)
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 500 µl of sterile water containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 10 µg/ml.
Reported Applications
WB
Western Blotting: To detect Human APRIL this biotin conjugate can be used at a concentration of 0.1-0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human APRIL is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human APRIL (Leinco Prod. No.: A413).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Sarah G. Hymowitz et al. (2004) J Mol Biol. 343: 283
2. (1998) Jürg Tschopp
3. Ruggero De Maria et al. (2004) Blood 104: 3169
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human APRIL (Leinco Prod. No.: A413)
Anti-Human APRIL (Antigen Aff. Pur.) (Leinco Prod. No.: A206)
APRIL (Extracellular Domain-2) Blocking Peptide (Leinco Prod. No.: A186)
Anti-Human APRIL (Extracellular Domain-1) (Leinco Prod. No.: A154)
Anti-Human APRIL (Extracellular Domain-2) (Leinco Prod. No.: A155)
APRIL (Extracellular Domain-1) Blocking Peptide (Leinco Prod. No.: A185)
Recombinant Mouse APRIL (A Proliferation-Inducing Ligand) (Leinco Prod. No.: A200)
UltraAvadin™ Reagents
Streptavadin Reagents
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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