Purified Recombinant Human APRIL (>98%)
Country of Origin
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Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human APRIL this biotin conjugate can be used at a concentration of 0.1-0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human APRIL is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:A413.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Human A Proliferation-Inducing Ligand (APRIL) recognizes Human APRIL. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
NCBI Gene Bank ID
References & Citations
1. Hymowitz, SG. et al. (2004) J Mol Biol. 343: 283
2. Tschopp, J. et al. (1998) J Exp Med. 188(6):1185-90.
3. De Maria, R. et al. (2004) Blood 104: 3169
Products are for research use only. Not for use in diagnostic or therapeutic procedures.