Purified Recombinant Human APRIL (>98%)
<0.1 EU/µg as determined by the LAL method
>95% by SDS Page and HPLC
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: Suitable for use at concentration of 0.1 µg/mL.
ELISA: This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody.
Flow Cytometry: Suitable for use at concentration of 2.5 µg/106 cells
Other Applications Reported In Literature ?
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human A Proliferating-Inducing Ligand (APRIL) recognizes Human APRIL. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
A proliferation-inducing ligand (APRIL) is a TNF-like cytokine that stimulates tumor cell growth.1 It is of low abundance in normal tissues.2 APRIL acts as a proliferative factor in megakaryocytopoiesis, a developmental hematopoietic process responsible for progenitor cell differentiation to megakaryoblasts and megakaryocytes, leading to platelet formation.3 APRIL may be implicated in the regulation of tumor cell growth.2
NCBI Gene Bank ID
References & Citations
1. Hymowitz, SG. et al. (2004) J Mol Biol. 343: 283
2. Tschopp, J. et al. (1998) J Exp Med. 188(6):1185-90.
3. De Maria, R. et al. (2004) Blood 104: 3169
Products are for research use only. Not for use in diagnostic or therapeutic procedures.