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CIDE-A (CT) (Human) Blocking Peptide
Blocking Peptide
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Size


Prod. No.:C1136
Conc.:0.2 mg/ml
Pkg. Size:50 µg
Storage:-20°C Detailed storage instructions below.
Description
Background:
Cell Death-Inducing DFF-Like Effector A peptide is applicable as a control peptide for blocking antibody binding in Western blotting.

Amino Acid Location
This peptide sequence is located at amino acids 200 to 217 of Human CIDE-A protein.
Formulation
This peptide is formulated in PBS pH 7.2 (0.01 M Sodium Phosphate, 0.13 M NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage and Stability
Store this peptide in working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
N, WB
Neutralization: The peptide may be used to neutralize the binding activity of Anti-Human CIDE-A (CT) (Leinco Prod. No.: C1292) by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He WW, Dixit VM. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J Biol Chem 1996;271:16720-4

2. Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N, Armstrong RC, Wang L, Trapani JA, Tomaselli KJ, Litwack G, Alnemri ES. The Ced-3/interleukin 1β converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2α are substrates for the apoptotic mediator CPP32. J Biol Chem 1996;271:27099-106

3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89

4. Pan G, O'Rourke K, Dixit VM. Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex. J Biol Chem 1998;273:5841-5 (RD1299)

5. Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J 1998;17:2526-33
Related Products
Anti-Human CIDE-A (CT) (Leinco Prod. No.: C1292)
CIDE-A (CT) (Mouse) Blocking Peptide (Leinco Prod. No.: C1147)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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