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Anti-Human Eotaxin-2
Purified No Carrier Protein
(CCL24, MPIF-2, CkB-6, SCYA24)
Monoclonal Antibody
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Size


Prod. No.:E139
Clone:61016
Isotype:Mouse IgG1
Pkg. Size:500 µg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human Eotaxin-2 recognizes Human Eotaxin-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
Eotaxin-2 is a CC chemokine that is a specific agonist for the chemokine receptor CCR3. It is produced by activated monocytes and T lymphocytes. Eotaxin-2 induces chemotaxis of eosinophils as well as basophils and the release of histamine and leukotriene C4 from basophils. Eotaxin-2 has the same efficacy as eotaxin, but is somewhat less potent.1 Additionally, Eotaxin-2 inhibits the proliferation of multipotential hematopoietic progenitor cells.

Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human CCL24
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, N, WB
Neutralization: This antibody is useful for neutralization of Human Eotaxin-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human Eotaxin-2 (at 100 ng/ml) is 0.2 - 0.8 µg/ml.
Western Blotting: To detect Human Eotaxin-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human Eotaxin-2 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Marco Baggiolini et al. (1997) J. Exp. Med. 185: 2171
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human Eotaxin-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E125)
Recombinant Human Eotaxin-2 (Leinco Prod. No.: E112)
Anti-Mouse Eotaxin-2 (Leinco Prod. No.: C1434)
Anti-Mouse Eotaxin-2 (Antigen Aff. Pur.) (Leinco Prod. No.: E143)
Anti-Human Eotaxin-2 (Antigen Aff. Pur.) (Leinco Prod. No.: E117)
Recombinant Mouse Eotaxin-2 (Leinco Prod. No.: E129)
Anti-Mouse Eotaxin-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E144)
Recombinant Mouse Eotaxin-2 (aa 27-119) (Leinco Prod. No.: E307)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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