Anti-Mouse Eotaxin-2 (Clone 106521)

Pricing & Details

Product No.C1434
Clone
106521
Protein
Eotaxin-2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CCL24, MPIF-2, CkB-6, SCYA24
Isotype
Rat
IgG2a
Applications
ELISA Cap
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1434-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
C1434-100 µg
100 µg
$379.00
In stock
Max:
Min: 1
Step: 1
C1434-500 µg
500 µg
$920.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
E. coli-Derived Recombinant Mouse CCL24
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse CCL24 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for rmCCL24 is approximately 5 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Capture: Suitable for use at concentration of 2-8 µg/mL. Use in combination with: Detection Reagent: Mouse CCL24/Eotaxin‑2/MPIF‑2 Biotinylated Antibody. Standard: Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 24-119) Protein.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse CCL24 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CCL24 (at 20 ng/ml) is 0.06 - 0.3 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Eotaxin-2 (Clone 106521) recognizes an epitope on Mouse Eotaxin-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Eotaxin-2 is a CC chemokine that is a specific agonist for the chemokine receptor CCR3. It is produced by activated monocytes and T lymphocytes. Eotaxin-2 induces chemotaxis of eosinophils as well as basophils and the release of histamine and leukotriene C4 from basophils. Eotaxin-2 has the same efficacy as eotaxin, but is somewhat less potent. 1
Additionally, Eotaxin-2 inhibits the proliferation of multipotential hematopoietic progenitor cells.

Antigen Details

Protein
PubMed

References & Citations

1. Baggiolini, M. et al. (1997) J. Exp. Med. 185: 2171
Elisa Sandwich Protocol
General Western Blot Protocol

Formats Available

Prod No.
Description
C1434
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Leinco Technologies uses cookies to improve your experience and our website service. By continuing to browse our website, you accept our cookie policy. Ok