Anti-Human Eotaxin-2 (Clone 61016)
Anti-Human Eotaxin-2 (Clone 61016)
Product No.: E139
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Clone 61016 Target Eotaxin-2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CCL24, MPIF-2, CkB-6, SCYA24 Isotype IgG1 Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen E. coli-Derived Recombinant Human CCL24 (Accession # AAB51135) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2829989 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human Eotaxin-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Eotaxin-2 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. ELISA Capture: Suitable for use at concentration of 2-8 µg/mL. Use in combination with: Detection Reagent: Human CCL24/Eotaxin‑2/MPIF‑2 Biotinylated Antibody. Standard: Recombinant Human CCL24/Eotaxin-2/MPIF-2 Protein. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human Eotaxin-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human Eotaxin-2 (at 100 ng/ml) is 0.2 - 0.8 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Eotaxin-2 recognizes Human Eotaxin-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Eotaxin-2 is a CC chemokine that is a specific agonist for the chemokine receptor CCR3. It is produced by activated monocytes and T lymphocytes. Eotaxin-2 induces chemotaxis of eosinophils as well as basophils and the release of histamine and leukotriene C4 from basophils. Eotaxin-2 has the same efficacy as eotaxin, but is somewhat less potent.1 Additionally, Eotaxin-2 inhibits the proliferation of multipotential hematopoietic progenitor cells. Antigen DetailsPubMed References & Citations1. Baggiolini, M. et al. (1997) J. Exp. Med. 185: 2171 Technical Protocols N  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
