E. coli-Derived Recombinant Human CCL24 (Accession # AAB51135)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human Eotaxin-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Eotaxin-2 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Capture: Suitable for use at concentration of 2-8 µg/mL. Use in combination with: Detection Reagent: Human CCL24/Eotaxin‑2/MPIF‑2 Biotinylated Antibody. Standard: Recombinant Human CCL24/Eotaxin-2/MPIF-2 Protein.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human Eotaxin-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human Eotaxin-2 (at 100 ng/ml) is 0.2 - 0.8 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human Eotaxin-2 recognizes Human Eotaxin-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Eotaxin-2 is a CC chemokine that is a specific agonist for the chemokine receptor CCR3. It is produced by activated monocytes and T lymphocytes. Eotaxin-2 induces chemotaxis of eosinophils as well as basophils and the release of histamine and leukotriene C4 from basophils. Eotaxin-2 has the same efficacy as eotaxin, but is somewhat less potent.1 Additionally, Eotaxin-2 inhibits the proliferation of multipotential hematopoietic progenitor cells.
References & Citations
1. Baggiolini, M. et al. (1997) J. Exp. Med. 185: 2171
Products are for research use only. Not for use in diagnostic or therapeutic procedures.