Anti-Mouse Eotaxin-2

E. coli-Derived Recombinant Mouse Eotaxin-2

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

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Neutralization: This antibody is useful for neutralization of Mouse Eotaxin-2 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse Eotaxin-2 (at 0.02 ng/ml) is 0.5 - 2.5 µg/ml.

Goat Anti-Mouse Eotaxin-2 recognizes Mouse Eotaxin-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Eotaxin-2 is a CC chemokine that is a specific agonist for the chemokine receptor CCR3. It is produced by activated monocytes and T lymphocytes. Eotaxin-2 induces chemotaxis of eosinophils as well as basophils and the release of histamine and leukotriene C4 from basophils. Eotaxin-2 has the same efficacy as eotaxin, but is somewhat less potent. ¹
Additionally, Eotaxin-2 inhibits the proliferation of multipotential hematopoietic progenitor cells.

1. Baggiolini, M. et al. (1997) J. Exp. Med. 185: 2171