Recombinant Human CD27

CD27 is a tumor necrosis factor receptor. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It exists as both a disulfide-linked dimer on the cell surface and as a soluble protein found in serum. It binds to ligand CD70 which is expressed on thymic stromal cells and a small subset of activated T cells and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

The biological activity of Human CD27 was determined by its ability to inhibit the proliferation of mouse T cells induced by recombinant mouse CD27 ligand (10 μg/ml, 100 μl/well) and a sub-optimal concentration of anti-CD3. The expected ED₅₀ is typically 0.75 - 3 μg/ml.

Fc Fusion Protein

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Thr21

Lyophilized

The predicted molecular weight of Recombinant Human CD27 is Mr 46.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 60-70 kDa.

46.5

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Van Oosterwijk, MF. et al. (2007) Int. Immunol. 19:713.
2. Nolte, MA. et al. (2009) Immunol. Rev. 229:216.