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Anti-Human Endocrine-Gland-Derived Vascular Endothelial Cell Growth Factor (EG-VEGF)

Purified No Carrier Protein

(Prokineticin, PROK1, PK1)

Monoclonal Antibody

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Size

500 µg ( $325.00 )

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Prod. No.:	E190
Clone:	188608
Isotype:	Mouse IgG₁
Pkg. Size:	500 µg
Storage:	-20°C to -70°C Detailed storage instructions below.

Description

Specificity:

Mouse Anti-Human Endocrine Gland-Derived Endothelial Growth Factor (EG-VEGF) (Clone 188608) recognizes an epitope on Human EG-VEGF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

Human EG-VEGF is a secreted angiogenetic mitogen growth factor expressed in the steroidogenic glands, ovary, testis, adrenal and placenta.

Background:

Endocrine Gland-Derived Vascular Endothelial Growth Factor (EG-VEGF) or Prokineticin-1 (PK-1) is a novel secreted protein expressed in various tissues including steroidogenic glands. Although structurally non-related to VEGF, EG-VEGF/PK-1 was shown to promote angiogenesis acting via two G-protein coupled receptors (PK-R1 and PK-R2). ¹ EG-VEGF is mitogen specific for the endothelium of steroidogenic glands. It induces proliferation, migration and fenestration in capillary endothelial cells derived from endocrine glands. EG-VEGF mRNA expression is localized to the steroid hormone-producing cells, i.e. Leydig cells of the testis and the specialized stroma in the ovary. ²
EG-VEGF increased the phosphorylation of Akt in a phosphatidylinositol 3-kinase-dependent manner and is a survival factor for ACE cells.
EG-VEGF, possibly through binding to a G-protein coupled receptor, results in the activation of MAPK p44/42 and phosphatidylinositol 3-kinase signaling pathways, leading to proliferation, migration, and survival of responsive endothelial cells. ³

Known Species Cross Reactivity: Hu+

Host Species

Mouse

Immunogen

Purified NSO cell derived Recombinant Human EG-VEGF

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Reconstitution

This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.

Endotoxin

<0.1 EU/µg as determined by the LAL method

Storage and Stability

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Reported Applications

ELISA Cap

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is Anti-Human EG-VEGF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E147) at a concentration of approximately 0.1-0.4 µg/ml. When used in conjunction with an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" (Leinco Prod. No.: T118), the sensitivity of this sandwich ELISA is 16 ng/well. A suggested standard for this assay is Recombinant Human EG-VEGF (Leinco Prod. No.: V108).

Each investigator should determine their own optimal working dilution for specific applications.

Country of Origin

USA

References

1. Rehovot, Israel et al. (2005) SSR
2. R Lin & F Peale et al. (2003) Endocrine Abstracts 5:S11
3. Napoleone Ferrara et al. (2002) J. Biol. Chem 277:8724