Anti-Human EG-VEGF (Clone 188608)
Anti-Human EG-VEGF (Clone 188608)
Product No.: E190
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Clone 188608 Target EG-VEGF Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Endocrine-Gland-Derived Vascular Endothelial Cell Growth Factor, Prokineticin, PROK1, PK1 Isotype IgG1 Applications ELISA Cap |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified NSO cell derived Recombinant Human EG-VEGF (Accession # P58294) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2829998 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:E147 at a concentration of approximately 0.1-0.4 µg/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is 16 ng/well. A suggested standard for this assay is PN:V108. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Endocrine Gland-Derived Endothelial Growth Factor (EG-VEGF) (Clone 188608) recognizes an epitope on Human EG-VEGF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen Distribution Human EG-VEGF is a secreted angiogenetic mitogen growth factor expressed in the steroidogenic glands, ovary, testis, adrenal and placenta. Background Endocrine Gland-Derived Vascular Endothelial Growth Factor (EG-VEGF) or Prokineticin-1 (PK-1) is a novel secreted protein expressed in various tissues including steroidogenic glands. Although structurally non-related to VEGF, EG-VEGF/PK-1 was shown to promote angiogenesis acting via two G-protein coupled receptors (PK-R1 and PK-R2). 1 EG-VEGF is mitogen specific for the endothelium of steroidogenic glands. It induces proliferation, migration and fenestration in capillary endothelial cells derived from endocrine glands. EG-VEGF mRNA expression is localized to the steroid hormone-producing cells, i.e. Leydig cells of the testis and the specialized stroma in the ovary. 2 EG-VEGF increased the phosphorylation of Akt in a phosphatidylinositol 3-kinase-dependent manner and is a survival factor for ACE cells. EG-VEGF, possibly through binding to a G-protein coupled receptor, results in the activation of MAPK p44/42 and phosphatidylinositol 3-kinase signaling pathways, leading to proliferation, migration, and survival of responsive endothelial cells. 3 Antigen DetailsPubMed References & Citations1. Meidan, R. et al. (2005) Endocrinology. 146(9):3950-8. 2. Ferrara, N. et al. (2002) Cold Spring Harb Symp Quant Biol.67:217-21. 3. Ferrara, N. et al. (2002) J Biol Chem. 277(10):8724-9. Technical Protocols |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
