Anti-Rat TIMP-1 (Clone 150911)
Anti-Rat TIMP-1 (Clone 150911)
Product No.: T444
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Clone 150911 Target TIMP-1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names EPO, CLGI, EPA, FLJ90373, HCI, TIMP, Tissue Inhibitor of Metalloproteinase 1 Isotype IgG2b Applications WB |
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Mouse Immunogen NS0-Derived Recombinant Rat TIMP-1 Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831944 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat TIMP-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat TIMP-1 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Rat Tissue Inhibitor of Metalloproteinase 1 (TIMP-1) (Clone 150911) recognizes an epitope on Rat TIMP-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Tissue Inhibitor of Metalloproteinases 1, also known as TIMP1 is a widely expressed, secreted protein that functions primarily to inhibit members of a large family of metalloproteinases (MPs). Because of the ability of TIMP-1 to inhibit MPs, it functions in many of the same pathophysiological processes as these enzymes, e.g. wound healing, ovulation, angiogenesis, and cancer cell metastasis. TIMP-1 can also stimulate proliferation and cellular anabolic processes.1 TIMP-1 is a novel negative regulator of HGF activity during liver regeneration.2 Overexpression of TIMP-1 could promote renal interstitial fibrosis through the inflammatory pathway, which might be partly induced by upregulating ICAM-1.3 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Denhardt, DT.et al. (2005) Breast Cancer Res Treat. 94: 185 2. Khokha, R. et al. (2005) Hepatology 41: 857 3. Chen, X. et al. (2008) Nephrology Dialysis Transportation 23: 1861 Technical ProtocolsCertificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.