Anti-Rat TIMP-1 (Clone 150911)

Anti-Rat TIMP-1 (Clone 150911)

Product No.: T444

[product_table name="All Top" skus="T444"]

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Clone
150911
Target
TIMP-1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
EPO, CLGI, EPA, FLJ90373, HCI, TIMP, Tissue Inhibitor of Metalloproteinase 1
Isotype
IgG2b
Applications
WB

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Antibody Details

Product Details

Reactive Species
Rat
Host Species
Mouse
Immunogen
NS0-Derived Recombinant Rat TIMP-1
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Rat TIMP-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat TIMP-1 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Mouse Anti-Rat Tissue Inhibitor of Metalloproteinase 1 (TIMP-1) (Clone 150911) recognizes an epitope on Rat TIMP-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Tissue Inhibitor of Metalloproteinases 1, also known as TIMP1 is a widely expressed, secreted protein that functions primarily to inhibit members of a large family of metalloproteinases (MPs). Because of the ability of TIMP-1 to inhibit MPs, it functions in many of the same pathophysiological processes as these enzymes, e.g. wound healing, ovulation, angiogenesis, and cancer cell metastasis. TIMP-1 can also stimulate proliferation and cellular anabolic processes.1 TIMP-1 is a novel negative regulator of HGF activity during liver regeneration.2 Overexpression of TIMP-1 could promote renal interstitial fibrosis through the inflammatory pathway, which might be partly induced by upregulating ICAM-1.3
PubMed
NCBI Gene Bank ID

References & Citations

1. Denhardt, DT.et al. (2005) Breast Cancer Res Treat. 94: 185
2. Khokha, R. et al. (2005) Hepatology 41: 857
3. Chen, X. et al. (2008) Nephrology Dialysis Transportation 23: 1861
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
T444
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.