Anti-Human TIMP-1 (Clone 63515)
Anti-Human TIMP-1 (Clone 63515)
Product No.: T462
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Clone 63515 Target TIMP-1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Tissue Inhibitor of Metalloproteinase 1, EPO, CLGI, EPA, FLJ90373, HCI, TIMP Isotype IgG2b Applications CyTOF® , ELISA Cap , FC , ICC , IHC FFPE , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human TIMP-1 (Accession # P01033) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831962 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T416 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T411. Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TIMP-1. A suggested method would be to stain cells expressing TIMP-1 with 0.25 µg per 1-5 x 105 cells in a total staining volume of ≤200 µl followed by PN:M1189. Western Blotting: To detect Human TIMP-1 this monoclonal antibody can be used at a concentration of 0.1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TIMP-1 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T411. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10-15 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Immunocytochemistry: Suitable for use at concentration of 3-25 µg/mL. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) (Clone 63515) recognizes an epitope on Human TIMP-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Tissue Inhibitor of Metalloproteinases 1, also known as TIMP1 is a widely expressed, secreted protein that functions primarily to inhibit members of a large family of metalloproteinases (MPs). Because of the ability of TIMP-1 to inhibit MPs, it functions in many of the same pathophysiological processes as these enzymes, e.g. wound healing, ovulation, angiogenesis, and cancer cell metastasis. TIMP-1 can also stimulate proliferation and cellular anabolic processes.1 TIMP-1 is a novel negative regulator of HGF activity during liver regeneration.2 Overexpression of TIMP-1 could promote renal interstitial fibrosis through the inflammatory pathway, which might be partly induced by upregulating ICAM-1.3 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Denhardt, DT.et al. (2005) Breast Cancer Res Treat. 94: 185 2. Khokha, R. et al. (2005) Hepatology 41: 857 3. Chen, X. et al. (2008) Nephrology Dialysis Transportation 23: 1861 Technical ProtocolsCyTOF®   ICC IHC FFPE  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
