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Anti-Human CXCL1
Purified No Carrier Protein
(GRO1, GROa, MGSA, MGSA Alpha, NAP-3, SCYB1)
Monoclonal Antibody
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Prod. No.:C1393
Clone:20326
Isotype:Mouse IgG2b
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CXCL1 (Clone 20326) recognizes an epitope on Human CXCL1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody exhibits approximately 20% cross-reactivity with rhCXCL2/GROβ and rhCXCL3/GROγ, and no cross-reactivity with other chemokines tested.

Background:
Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).1 CXCL1 is expressed by macrophages, neutrophils and epithelial cells.2 CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.3,4

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human CXCL1 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, FC, N, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is Anti-Human CXCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G145) at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is Recombinant Human CXCL1 (Leinco Prod. No.: G125).
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCL1. A suggested method would be to stain cells expressing CXCL1 with 0.25 µg per 1 - 5 x 105 cells in a total staining volume of ≤ 200 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189).
Neutralization: This antibody is useful for neutralization of Human CXCL1 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CXCL1 (at 10 ng/ml) is 3-15 µg/ml.
Western Blotting: To detect Human CXCL1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human CXCL1 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human CXCL1 (Leinco Prod. No.: G125).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Sager R et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87: 7732
2. Grotendorst GR et al. (1990) Mol. Cell. Biol. 10: 5596
3. Miller RH et al. (2002) Cell 110: 373
4. Richmond A et al. (2000) J. Invest. Dermatol. 115: 234
Related Products
Anti-Human CXCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G145)
Recombinant Human CXCL1 (Leinco Prod. No.: G125)
Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189)
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Rat CXCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G144)
Anti-Mouse CXCL1 (Antigen Aff. Pur.) (Leinco Prod. No.: K125)
Anti-Rat CXCL1 (Antigen Aff. Pur.) (Leinco Prod. No.: G121)
Anti-Mouse KC-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: K133)
Recombinant Mouse CXCL1 (aa 29 - 96) Truncated Form (Leinco Prod. No.: K134)
Anti-Mouse CXCL1 (Leinco Prod. No.: K135)
Anti-Human CXCL1 (Leinco Prod. No.: C1394)
Anti-Mouse CXCL1 (Leinco Prod. No.: C1420)
Anti-Mouse CXCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: K124)
Rat CINC-1/CXCL1 ELISA Development Kit (Leinco Prod. No.: C1608)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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