Anti-Rat CXCL1

Anti-Rat CXCL1

Product No.: G121

[product_table name="All Top" skus="G121"]

Protein
CXCL1
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
GRO1, GROa, MGSA, MGSA Alpha, NAP-3, SCYB1
Applications
IHC FF
,
N
,
WB

Product Size

Antibody Details

Product Details

Reactivity Species
Rat
Host Species
Goat
Immunogen
Purified Recombinant Rat GRO/KC (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: Suitable for use at concentration of 0.1 µg/mL.
Additional Applications Reported In Literature ?
Immunohistochemistry: Suitable for use at concentration of 5-15 µg/mL.
Neutralization: Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.15-0.75 µg/mL in the presence of 4 ng/mL Recombinant Rat CXCL1/GRO alpha /KC/CINC‑1.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Rat CXCL1 recognizes Rat CXCL1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).1 CXCL1 is expressed by macrophages, neutrophils and epithelial cells.2 CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.3,4

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Sager, R. et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87: 7732
2. Grotendorst, GR. et al. (1990) Mol. Cell. Biol. 10: 5596
3. Miller, RH. et al. (2002) Cell 110: 373
4. Richmond, A. et al. (2000) J. Invest. Dermatol. 115: 234
IHC
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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