Recombinant Human CXCL1

Recombinant Human CXCL1

Product No.: G125

[product_table name="All Top" skus="G125"]

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Alternate Names
GRO1, GROa, MGSA, MGSA Alpha, NAP-3, SCYB1, FSP
Product Type
Recombinant Protein
Expression Host
E. coli Cells
Species
Human

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Data

Human CXCL1 Bioactivity Data
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Background

Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).1 CXCL1 is expressed by macrophages, neutrophils and epithelial cells.2 CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.3,4

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human GRO/MGSA was determined by its ability to induce myeloperoxidase release from cytochalasin treated neutrophils (Schröder, J. et al., 1987, J. Immunol. 139:3474), or chemotaxis of mouse BaF/3 cells transfected with hCXCR2. The expected ED<sub>50</sub> for these effects are typically 0.15 - 0.3 μg/ml or 1 - 4 ng/ml, respectively.
Protein Accession No.
P09341
Amino Acid Sequence
asvate lrcqclqtlq gihpkniqsv nvkspgphca qteviatlkn grkaclnpas pivkkiiekm lnsdksn
N-terminal Sequence Analysis
Ala35
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CXCL1 is Mr 7.9 kDa.
Predicted Molecular Mass
7.9
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Sager, R. et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87: 7732
2. Grotendorst, GR. et al. (1990) Mol. Cell. Biol. 10: 5596
3. Miller, RH. et al. (2002) Cell 110: 373
4. Richmond, A. et al. (2000) J. Invest. Dermatol. 115: 234

Certificate of Analysis

IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.