Recombinant Mouse KC/CXCL1

CXCL1 is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).¹ CXCL1 is expressed by macrophages, neutrophils and epithelial cells.² CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.^3,4

The biological activity of Mouse KC aa 20 - 96 was determined by its ability to induce myeloperoxidase release from cytochalasin B treated neutrophils (Schröder, J.M., et al., 1987, J. Immunol. 139:3474) or chemotaxis of mouse BaF/3 hCXCR2 transfected cells. . The expected ED₅₀ for these effects are typically 0.3 - 0.9 μg/ml or 3 - 15 ng/ml, respectively.

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Arg20

Lyophilized

The predicted molecular weight of Recombinant Mouse KC is Mr 8 kDa.

8

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Sager, R. et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87: 7732
2. Grotendorst, GR. et al. (1990) Mol. Cell. Biol. 10: 5596
3. Miller, RH. et al. (2002) Cell 110: 373
4. Richmond, A. et al. (2000) J. Invest. Dermatol. 115: 234