Anti-Rat CXCL1 – Biotin

Anti-Rat CXCL1 – Biotin

Product No.: G144

[product_table name="All Top" skus="G144"]

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Target
CXCL1
Product Type
Polyclonal Antibody
Alternate Names
GRO1, GROa, MGSA, MGSA Alpha, NAP-3, SCYB1
Applications
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Rat
Host Species
Goat
Immunogen
Purified Recombinant Rat CXCL1 (Accession # P14095)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Rat GRO/KC this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Rat GRO/KC is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Rat CXCL1 recognizes Rat CXCL1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).1 CXCL1 is expressed by macrophages, neutrophils and epithelial cells.2 CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.3,4

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Sager, R. et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87: 7732
2. Grotendorst, GR. et al. (1990) Mol. Cell. Biol. 10: 5596
3. Miller, RH. et al. (2002) Cell 110: 373
4. Richmond, A. et al. (2000) J. Invest. Dermatol. 115: 234
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.