Anti-Mouse CXCL1 (Clone 48415)

Pricing & Details


Product No.C1420 Clone 48415 Protein CXCL1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names GRO1, GROa, MGSA, MGSA Alpha, NAP-3, SCYB1 Isotype Rat IgG2a Applications ELISA Cap , N , WB Submit A Review	Printable Datasheet Printable SDS

Prod No.	Size	Price	Avail.	Qty	Add to cart
C1420-25 µg	25 µg	$230.00	In stock	Max: Min: 1 Step: 1	Add to Cart
C1420-100 µg	100 µg	$440.00	In stock	Max: Min: 1 Step: 1	Add to Cart
C1420-500 µg	500 µg	$1,025.00	In stock	Max: Min: 1 Step: 1	Add to Cart


Antibody Details Product Details Reactivity Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse CXCL1 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828480 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:K124 at a concentration of approximately 0.1 - 0.4 µg/ml. A suggested standard for this assay is PN:K144. Western Blotting: To detect Mouse CXCL1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CXCL1 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Other Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse CXCL1 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse CXCL1 (at 30 ng/ml) is 0.05 - 0.25 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Rat Anti-Mouse CXCL1 (Clone 48415) recognizes an epitope on Mouse CXCL1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).¹ CXCL1 is expressed by macrophages, neutrophils and epithelial cells.² CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.^3,4 Antigen Details Protein CXCL1 PubMed CXCL1 References & Citations 1. Sager, R. et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87: 7732 2. Grotendorst, GR. et al. (1990) Mol. Cell. Biol. 10: 5596 3. Miller, RH. et al. (2002) Cell 110: 373 4. Richmond, A. et al. (2000) J. Invest. Dermatol. 115: 234 Technical Protocols

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Purified Recombinant Mouse CXCL1 (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828480

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:K124 at a concentration of approximately 0.1 - 0.4 µg/ml. A suggested standard for this assay is PN:K144.
Western Blotting: To detect Mouse CXCL1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CXCL1 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Other Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Mouse CXCL1 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse CXCL1 (at 30 ng/ml) is 0.05 - 0.25 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse CXCL1 (Clone 48415) recognizes an epitope on Mouse CXCL1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine with mitogenic properties belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).¹ CXCL1 is expressed by macrophages, neutrophils and epithelial cells.² CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing.^3,4

Antigen Details

Protein

CXCL1

PubMed

CXCL1

References & Citations

1. Sager, R. et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87: 7732
2. Grotendorst, GR. et al. (1990) Mol. Cell. Biol. 10: 5596
3. Miller, RH. et al. (2002) Cell 110: 373
4. Richmond, A. et al. (2000) J. Invest. Dermatol. 115: 234

Prod No.	Description
G121	Anti-Rat CXCL1
K135	Anti-Mouse CXCL1 (Clone 124014)
I-202	Recombinant Human CXCL10
C1393	Anti-Human CXCL1 (Clone 20326)
C1394	Anti-Human CXCL1 (Clone 31716)
K125	Anti-Mouse CXCL1
C1420	Anti-Mouse CXCL1 (Clone 48415)
G126	Recombinant Rat CXCL1/KC
G125	Recombinant Human CXCL1
K133	Anti-Mouse KC-Biotin
K114	Recombinant Mouse KC/CXCL1
G144	Anti-Rat CXCL1 – Biotin
G145	Anti-Human CXCL1-Biotin
K124	Anti-Mouse CXCL1- Biotin
G718	Human GRO alpha/CXCL1 ELISA Development Kit
G700	Rat CXCL1/CINC-1 ELISA Development Kit
C1608	Rat CINC-1/CXCL1 ELISA Development Kit

Formats Available

Prod No.	Description
C1420	Anti-Mouse CXCL1 (Clone 48415)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.