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Product Datasheet

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Goat F(ab')₂ Anti-Rabbit IgG (H&L)

Allophycocyanin (APC)

Secondary Antibody

Adsorbed Against: Hu., Bv.

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Size

0.5 mg ( $266.00 )

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Prod. No.:	R128
Conc.:	1.0 mg/ml
Pkg. Size:	0.5 mg
Storage:	2-8°C Detailed storage instructions below.

Description

Specificity:

Goat F(ab')₂ Anti-Rabbit IgG (H&L) recognizes Rabbit IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:

Allophycocyanin (APC) has a molecular weight of 104 kDa and is purified from green algae. APC is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

Preparation:

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Host Species

Goat

Immunogen

Purified Rabbit IgG (H+L) (>98%)

Formulation

This secondary antibody is formulated in 0.01M phosphate buffered saline (PBS) pH 7.4 containing 1.0% BSA and 0.09% sodium azide as a preservative.

Expiration Date

One year from date of receipt.

Applications and Suggested Working Dilutions

This secondary reagent has been optimally manufactured to detect primary rabbit antibodies. Leinco Technologies uses F(ab')₂ fragments to eliminate Fc receptor binding during use in immunohistochemistry, and flow cytometry. Leinco Technologies suggests using 1 microgram to stain 1 X 106 cells in flow cytometry. However, we suggest that each investigator determine their own optimal titer for other specific applications. High concentrations of glycerol such as contained in some mounting mediums will quench the fluorescent intensity of Allophycocyanin and other phycobilliproteins.

Country of Origin

USA