Pricing & Details


Product Type Secondary Antibody Submit A Review	Printable Datasheet Printable SDS

Prod No.	Size	Price	Avail.	Qty	Add to cart
H217-1.0 mg	1.0 mg	$75.00	In stock		Add to Cart

Antibody Details

Reactivity Species

Human

Host Species

Goat

Immunogen

Purified Human IgG (H&L) (>98%)

Product Concentration

1.0 mg/ml

Formulation

This secondary antibody is formulated in 0.01M phosphate buffered saline (PBS) pH 7.4 containing 1.0% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This secondary antibody conjugate should be stored at 2-8°C. Do not freeze.

Expiration Date

One year from date of receipt.

Working Concentration

IHC: 1:100

Applications and Recommended Usage ?
(Quality Tested by Leinco)

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.

IHC (NBF/Par.): This antibody may be used to stain nuclear membranes in cells, tissue preparations or subcellular fractions. The antibody works well with cells and tissue sections fixed with 2% paraformaldehyde and requires no dilution. Tonsil is the recommended positive control tissue.

Flow Cytometry: PN:A106

Western Blotting: To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents such as PN:M114 and a chromogenic substrate such as PN:T343, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.

Excitation Laser

Blue Laser (488 nm)

Specificity

Goat Whole Molecule Anti-Human IgG (H&L) recognizes Human IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein

IgG (H&L)

Prod No.	Description
H264	Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms.) – Purified
M1185	Goat Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – AMCA
M111	Goat Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – Purified
R115	Goat Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – HRP
R113	Goat Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – Purified
R114	Goat Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – FITC
H215	Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms.) – HRP
M113	Goat Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – FITC
M114	Goat Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – HRP
M1199	Goat Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – Texas Red
R102	Goat F(ab')2 Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – FITC
M102	Goat F(ab')2 Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – FITC
H217	Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms.) – FITC
M1259	Goat Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – PE
R117	Goat F(ab')₂ Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – Purified
R119	Goat F(ab')₂ Anti-Rat IgG (H&L) (Adsorbed Against: Ms., Hu.) – Purified
H554	Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms., Bv., Hs.) – Purified
M103	Goat F(ab’)2 Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – HRP
M105	Goat F(ab’)₂ Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – Biotin
M112	Goat F(ab')₂ Anti-Mouse IgG (H&L) (Adsorbed Against: Hu.) – Purified

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms.) – FITC