Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms.) – FITC
Pricing & Details
Antibody DetailsProduct DetailsReactivity Species Human Host Species Goat Immunogen Purified Human IgG (H&L) (>98%) Product Concentration 0.2 mg/ml Formulation This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This secondary antibody conjugate should be stored at 2-8°C. Do not freeze. Expiration Date One year from date of receipt. Country of Origin USA Working Concentration IHC: 1:100 Applications and Recommended Usage ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml. IHC (NBF/Par.): This antibody may be used to stain nuclear membranes in cells, tissue preparations or subcellular fractions. The antibody works well with cells and tissue sections fixed with 2% paraformaldehyde and requires no dilution. Tonsil is the recommended positive control tissue. Flow Cytometry: PN:A106 Western Blotting: To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents such as PN:M114 and a chromogenic substrate such as PN:T343, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Excitation Laser Blue Laser (490 nm) DescriptionSpecificity Goat Whole Molecule Anti-Human IgG (H&L) recognizes Human IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity. Antigen DetailsProtein PubMed |
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