Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms.) – FITC

Pricing & Details

Product No.H217
Protein
IgG (H&L)
Formats AvailableView All
Product Type
Secondary Antibody
Prod No.
Size
Price
Avail.
Qty
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H217-1.0 mg
1.0 mg
$75.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Goat
Immunogen
Purified Human IgG (H&L) (>98%)
Product Concentration
1.0 mg/ml
Formulation
This secondary antibody is formulated in 0.01M phosphate buffered saline (PBS) pH 7.4 containing 1.0% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This secondary antibody conjugate should be stored at 2-8°C. Do not freeze.
Expiration Date
One year from date of receipt.
Country of Origin
USA
Applications and Recommended Usage
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.

IHC (NBF/Par.): This antibody may be used to stain nuclear membranes in cells, tissue preparations or subcellular fractions. The antibody works well with cells and tissue sections fixed with 2% paraformaldehyde and requires no dilution. Tonsil is the recommended positive control tissue.

Flow Cytometry: PN:A106

Western Blotting: To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents such as PN:M114 and a chromogenic substrate such as PN:T343, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.
Working Concentration
IHC: 1:100
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Excitation Laser
Blue Laser (488 nm)

Description

Specificity
Goat Whole Molecule Anti-Human IgG (H&L) recognizes Human IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein
PubMed
Products are for research use only. Not for use in diagnostic or therapeutic procedures.