Pricing & Details


Product No.H217 Protein IgG (H&L) Formats AvailableView All Product Type Secondary Antibody References and Citations Submit A Review	Printable Datasheet Printable SDS

Prod No.	Size	Price	Avail.	Qty	Add to cart
H217-1.0 mg	1.0 mg	$75.00	In stock	Max: Min: 1 Step: 1	Add to Cart

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

Purified Human IgG (H&L) (>98%)

Product Concentration

1.0 mg/ml

Formulation

This secondary antibody is formulated in 0.01M phosphate buffered saline (PBS) pH 7.4 containing 1.0% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This secondary antibody conjugate should be stored at 2-8°C. Do not freeze.

Expiration Date

One year from date of receipt.

Country of Origin

USA

Applications and Recommended Usage

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.

IHC (NBF/Par.): This antibody may be used to stain nuclear membranes in cells, tissue preparations or subcellular fractions. The antibody works well with cells and tissue sections fixed with 2% paraformaldehyde and requires no dilution. Tonsil is the recommended positive control tissue.

Flow Cytometry: PN:A106

Western Blotting: To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents such as PN:M114 and a chromogenic substrate such as PN:T343, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.

Working Concentration

IHC: 1:100

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Excitation Laser

Blue Laser (488 nm)

Description

Specificity

Goat Whole Molecule Anti-Human IgG (H&L) recognizes Human IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein

IgG (H&L)

PubMed

IgG (H&L)

Prod No.	Description
H264	Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms.) – Purified
M1185	Goat Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – AMCA
M111	Goat Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – Purified
R115	Goat Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – HRP
R113	Goat Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – Purified
R114	Goat Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – FITC
H215	Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms.) – HRP
M113	Goat Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – FITC
M114	Goat Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – HRP
M1199	Goat Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – Texas Red
R102	Goat F(ab')2 Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – FITC
M102	Goat F(ab')2 Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – FITC
H217	Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms.) – FITC
M1259	Goat Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – PE
R117	Goat F(ab')₂ Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – Purified
R119	Goat F(ab')₂ Anti-Rat IgG (H&L) (Adsorbed Against: Ms., Hu.) – Purified
H554	Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms., Bv., Hs.) – Purified
M103	Goat F(ab’)2 Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – HRP
M105	Goat F(ab’)₂ Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – Biotin
M112	Goat F(ab')₂ Anti-Mouse IgG (H&L) (Adsorbed Against: Hu.) – Purified

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms.) – FITC