Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms.) – FITC

Pricing & Details

  
Product Type
Secondary Antibody
Prod No.
Size
Price
Avail.
Qty
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H217-1.0 mg
1.0 mg
$75.00
In stock
  

Antibody Details

Reactivity Species
Human
Host Species
Goat
Immunogen
Purified Human IgG (H&L) (>98%)
Product Concentration
1.0 mg/ml
Formulation
This secondary antibody is formulated in 0.01M phosphate buffered saline (PBS) pH 7.4 containing 1.0% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This secondary antibody conjugate should be stored at 2-8°C. Do not freeze.
Expiration Date
One year from date of receipt.
Working Concentration
IHC: 1:100
Applications and Recommended Usage ?
(Quality Tested by Leinco)
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.

IHC (NBF/Par.): This antibody may be used to stain nuclear membranes in cells, tissue preparations or subcellular fractions. The antibody works well with cells and tissue sections fixed with 2% paraformaldehyde and requires no dilution. Tonsil is the recommended positive control tissue.

Flow Cytometry: PN:A106

Western Blotting: To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents such as PN:M114 and a chromogenic substrate such as PN:T343, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.
Excitation Laser
Blue Laser (488 nm)
Specificity
Goat Whole Molecule Anti-Human IgG (H&L) recognizes Human IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein
IgG (H&L)
  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.