Goat Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – Purified

Pricing & Details

  
Product Type
Secondary Antibody
Prod No.
Size
Price
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M111-1.0 mg
1.0 mg
$61.00
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Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Goat
Immunogen
Purified Mouse IgG (H&L) (>98%)
Product Concentration
1.0 mg/ml
Formulation
This secondary antibody is aseptically packaged and formulated in 0.01M phosphate buffered saline (PBS) pH 7.4.
Storage and Handling
This secondary antibody conjugate should be stored for up to 4 weeks at 2-8°C. However, for long-term storage, aliquot into working volumes without dilution and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Expiration Date
One year from date of receipt
Country of Origin
USA
Applications and Recommended Usage
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.

IHC (NBF/Par.): This antibody may be used to stain nuclear membranes in cells, tissue preparations or subcellular fractions. The antibody works well with cells and tissue sections fixed with 2% paraformaldehyde and requires no dilution. Tonsil is the recommended positive control tissue.

Flow Cytometry: PN:A106

Neutralization: The peptide may be used to neutralize the binding activity of PN:Z100 by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.

Western Blotting: To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents such as PN:M114 and a chromogenic substrate such as PN:T343, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse IgG (H&L) recognizes Mouse IgG heavy and light chains. This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.
Protein
IgG (H&L)

References & Citations

  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.