Goat Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – HRP

Pricing & Details

  
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M114-0.5 mg
0.5 mg
$67.00
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Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Goat
Immunogen
Purified Mouse IgG (H&L)
Product Concentration
1.0 mg/ml
Formulation
This secondary antibody is formulated in 0.01M phosphate buffered saline (PBS) pH 7.4 containing 1.0% BSA and 0.01% thimerosal. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)
Storage and Handling
This secondary antibody should be stored at 2-8°C. For long term storage, add 50% volume for volume glycerol and store at -20°C.
Expiration Date
One year from date of receipt
Country of Origin
USA
See directions on lot specific datasheets, as information may periodically change.
Working Concentration
WB: 1:5,000 - 1:100,000
WB Chemiluminescent: 1:10,000 - 1:200,000
ELISA: 1:5,000 - 1:100,000
IHC: 1:500 - 1:5,000
Applications and Recommended Usage ?
(Quality Tested by Leinco)
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.

IHC (NBF/Par.): This antibody may be used to stain nuclear membranes in cells, tissue preparations or subcellular fractions. The antibody works well with cells and tissue sections fixed with 2% paraformaldehyde and requires no dilution. Tonsil is the recommended positive control tissue.

Flow Cytometry: PN:A106

Neutralization: The peptide may be used to neutralize the binding activity of PN:Z100 by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.

Western Blotting: To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents such as PN:M114 and a chromogenic substrate such as PN:T343, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.
Each investigator should determine their own optimal working dilution for specific applications.

Description

Specificity
Goat Anti-Mouse IgG (H&L) recognizes Mouse IgG heavy and light chains. This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein
IgG (H&L)

References & Citations

1. Pang, JS et al.(2017) J Mol Med (Berl).95(3):323-333. PubMed
  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.